Overview

  • Product name

  • Description

    Rabbit polyclonal to PRAK/MK5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to Human PRAK/MK5 aa 423-473.
    Database link: NP_620777.1

  • Positive control

    • Whole cell lysate from HeLa and 293T cells.
  • General notes

     This product was previously labelled as PRAK

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Mediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localization

    Cytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Images

  • All lanes : Anti-PRAK/MK5 antibody (ab93800) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK/MK5 with ab93800 at 1/200 (1µg/ml). Detection: DAB.

  • Detection of PRAK/MK5 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab93800 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93800.
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