Key features and details
- Rabbit polyclonal to PRAK/MK5
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRAK/MK5 antibody
See all PRAK/MK5 primary antibodies
DescriptionRabbit polyclonal to PRAK/MK5
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat
Synthetic peptide corresponding to Human PRAK/MK5 aa 423-473.
Database link: NP_620777.1
- Whole cell lysate from HeLa and 293T cells.
This product was previously labelled as PRAK
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMediates stress-induced small heat shock protein 27 phosphorylation.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
modificationsPhosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
Cellular localizationCytoplasm. Nucleus. Also observed in the nucleus.
- Information by UniProt
- MAP kinase-activated protein kinase 5 antibody
- MAPK-activated protein kinase 5 antibody
- MAPK5_HUMAN antibody
All lanes : Anti-PRAK/MK5 antibody (ab93800) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK/MK5 with ab93800 at 1/200 (1µg/ml). Detection: DAB.
Detection of PRAK/MK5 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab93800 has not yet been referenced specifically in any publications.