Anti-PRAK/MK5 (phospho T182) antibody (ab138668)
Key features and details
- Rabbit polyclonal to PRAK/MK5 (phospho T182)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAK/MK5 (phospho T182) antibody
See all PRAK/MK5 primary antibodies -
Description
Rabbit polyclonal to PRAK/MK5 (phospho T182) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human PRAK/MK5 (internal sequence) (phospho T182).
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Positive control
- WB: K562 cell lysate from cells treated with sodium orthovanadate. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 54 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates stress-induced small heat shock protein 27 phosphorylation. -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12. -
Cellular localization
Cytoplasm. Nucleus. Also observed in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8550 Human
- Entrez Gene: 17165 Mouse
- Omim: 606723 Human
- SwissProt: Q8IW41 Human
- SwissProt: O54992 Mouse
- Unigene: 413901 Human
- Unigene: 272206 Mouse
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Alternative names
- MAP kinase-activated protein kinase 5 antibody
- MAPK-activated protein kinase 5 antibody
- MAPK5_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for PRAK/MK5 (phospho T182) using ab138668 at 1/50 dilution. The right panel shows the effect of blocking with the immunozing phospho-peptide.
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Anti-PRAK/MK5 (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells treated with 0.3 nM sodium orthovanadate for 40 minutes) at 30 µg
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab138668 has been referenced in 3 publications.
- Bogucka K et al. ERK3/MAPK6 controls IL-8 production and chemotaxis. Elife 9:N/A (2020). PubMed: 32314963
- Zhao YT et al. PRAK plays a pivotal role in protecting the heart against ischemia/ reperfusion injury and myocardial infarction. Am J Physiol Cell Physiol N/A:N/A (2019). PubMed: 31291142
- Nawaito SA et al. MK5 haplodeficiency attenuates hypertrophy and preserves diastolic function during remodeling induced by chronic pressure overload in the mouse heart. Am J Physiol Heart Circ Physiol 313:H46-H58 (2017). PubMed: 28432058