Overview

  • Product name

    Anti-PRAK/MK5 (phospho T182) antibody
    See all PRAK/MK5 primary antibodies
  • Description

    Rabbit polyclonal to PRAK/MK5 (phospho T182)
  • Host species

    Rabbit
  • Specificity

    ab138668 detects endogenous levels of PRAK/MK5 only when phosphorylated at T182.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PRAK/MK5 (internal sequence).

  • Positive control

    • WB: K562 cell lysate from cells treated with sodium orthovanadate. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as PRAK

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Mediates stress-induced small heat shock protein 27 phosphorylation.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • Cellular localization

    Cytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for PRAK/MK5 (phospho T182) using ab138668 at 1/50 dilution. The right panel shows the effect of blocking with the immunozing phospho-peptide.

  • Anti-PRAK/MK5 (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells treated with 0.3 nM sodium orthovanadate for 40 minutes) at 30 µg

    Predicted band size: 54 kDa

References

This product has been referenced in:

  • Zhao YT  et al. PRAK plays a pivotal role in protecting the heart against ischemia/ reperfusion injury and myocardial infarction. Am J Physiol Cell Physiol N/A:N/A (2019). Read more (PubMed: 31291142) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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