Overview

  • Product name

    Anti-PRAME antibody - C-terminal
    See all PRAME primary antibodies
  • Description

    Rabbit polyclonal to PRAME - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PRAME aa 476-502 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P78395

  • Positive control

    • K562 cell lysate; Human testis tissue; K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 57 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
  • Tissue specificity

    Expressed in testis. Detected in samples of kidney, brain and skin.
  • Sequence similarities

    Belongs to the PRAME family.
    Contains 4 LRR (leucine-rich) repeats.
  • Cellular localization

    Nucleus. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930534P07Rik antibody
    • Cancer/testis antigen 130 antibody
    • CT130 antibody
    • MAPE antibody
    • Melanoma antigen preferentially expressed in tumors antibody
    • OIP 4 antibody
    • OIP-4 antibody
    • OIP4 antibody
    • OPA interacting protein 4 antibody
    • Opa interacting protein OIP4 antibody
    • OPA-interacting protein 4 antibody
    • PRAME antibody
    • PRAME_HUMAN antibody
    • Preferentially expressed antigen in melanoma antibody
    • Preferentially expressed antigen of melanoma antibody
    • RP23-250F8.3 antibody
    see all

Images

  • Anti-PRAME antibody - C-terminal (ab135600) at 1/50 dilution + K562 cell lysate at 35 µg

    Predicted band size: 57 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human testis tissue labelling PRAME with ab135600 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow cytometric analysis of K562 cells labelling PRAME with ab135600 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC conjugated Goat anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Malchenko S  et al. Characterization of brain tumor initiating cells isolated from an animal model of CNS primitive neuroectodermal tumors. Oncotarget 9:13733-13747 (2018). Read more (PubMed: 29568390) »
See 1 Publication for this product

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