Anti-PRAS40 antibody - C-terminal (ab181408)
Key features and details
- Rabbit polyclonal to PRAS40 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAS40 antibody - C-terminal
See all PRAS40 primary antibodies -
Description
Rabbit polyclonal to PRAS40 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PRAS40 (C terminal). Peptide corresponds to 19 amino acids (NP_115751).
Database link: Q96B36-3 -
Positive control
- WB: Human brain tissue lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. |
Target
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Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. -
Tissue specificity
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293). -
Post-translational
modificationsPhosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. -
Cellular localization
Cytoplasm > cytosol. Found in the cytosolic fraction of the brain. - Information by UniProt
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Database links
- Entrez Gene: 84335 Human
- Omim: 610221 Human
- SwissProt: Q96B36 Human
- Unigene: 515542 Human
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Alternative names
- 40 kDa proline rich AKT substrate antibody
- 40 kDa proline-rich AKT substrate antibody
- AKT1 S1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab181408 has not yet been referenced specifically in any publications.