Overview

  • Product name

    Anti-PRAS40 antibody - C-terminal
    See all PRAS40 primary antibodies
  • Description

    Rabbit polyclonal to PRAS40 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PRAS40 (C terminal). Peptide corresponds to 19 amino acids (NP_115751).
    Database link: Q96B36-3

  • Positive control

    • Rat brain tissue and Human brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab181408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa.

Target

  • Function

    Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  • Tissue specificity

    Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
  • Post-translational
    modifications

    Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • Cellular localization

    Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa proline rich AKT substrate antibody
    • 40 kDa proline-rich AKT substrate antibody
    • AKT1 S1 antibody
    • AKT1 substrate 1 (proline rich) antibody
    • AKT1 substrate 1 antibody
    • AKT1S 1 antibody
    • AKT1S1 antibody
    • AKTS1_HUMAN antibody
    • Lobe antibody
    • MGC2865 antibody
    • PRAS 40 antibody
    • PRAS antibody
    • PRAS40 antibody
    • Proline rich akt substrate antibody
    • Proline rich Akt substrate 40 kDa antibody
    • Proline-rich AKT1 substrate 1 antibody
    see all

Images

  • Lane 1 : Anti-PRAS40 antibody - C-terminal (ab181408) at 1 µg/ml
    Lane 2 : Anti-PRAS40 antibody - C-terminal (ab181408) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue, labeling PRAS40 using ab181408 at 2.5 μg/mL.

  • Immunofluorescence analysis of rat brain tissue, labeling PRAS40 using ab181408 at 20 μg/mL.

References

ab181408 has not yet been referenced specifically in any publications.

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