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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Anti-PRAS40 (phospho S183) antibody (ab226994)

  • Datasheet
  • SDS
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Western blot - Anti-PRAS40 (phospho S183) antibody (ab226994)

    Key features and details

    • Rabbit polyclonal to PRAS40 (phospho S183)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-PRAS40 (phospho S183) antibody
      See all PRAS40 primary antibodies
    • Description

      Rabbit polyclonal to PRAS40 (phospho S183)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human PRAS40 (C terminal) (phospho S183). The exact sequence is proprietary. (Carrier-protein conjugated).
      Database link: P00533

    • Positive control

      • WB: HEK-293T, starved for 16 hours, whole cell extract.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Kinases/Phosphatases
      • Other
      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Kinases
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab226994 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

    Target

    • Function

      Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
    • Tissue specificity

      Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
    • Post-translational
      modifications

      Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
    • Cellular localization

      Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
    • Target information above from: UniProt accession Q96B36 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 84335 Human
      • Omim: 610221 Human
      • SwissProt: Q96B36 Human
      • Unigene: 515542 Human
      • Alternative names

        • 40 kDa proline rich AKT substrate antibody
        • 40 kDa proline-rich AKT substrate antibody
        • AKT1 S1 antibody
        • AKT1 substrate 1 (proline rich) antibody
        • AKT1 substrate 1 antibody
        • AKT1S 1 antibody
        • AKT1S1 antibody
        • AKTS1_HUMAN antibody
        • Lobe antibody
        • MGC2865 antibody
        • PRAS 40 antibody
        • PRAS antibody
        • PRAS40 antibody
        • Proline rich akt substrate antibody
        • Proline rich Akt substrate 40 kDa antibody
        • Proline-rich AKT1 substrate 1 antibody
        see all

      Images

      • Western blot - Anti-PRAS40 (phospho S183) antibody (ab226994)
        Western blot - Anti-PRAS40 (phospho S183) antibody (ab226994)
        All lanes : Anti-PRAS40 (phospho S183) antibody (ab226994) at 1/1000 dilution

        Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract
        Lane 2 : HEK-293T, starved for 16 hours, and treated with 100 nM Rapamycin treated for 30 minutes whole cell extract
        Lane 3 : HEK-293T, starved for 16 hours and treated with 100 nM insulin for 30 minutes, whole cell extract
        Lane 4 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin and 100 nM insulin for 30 minutes, whole cell extract

        Lysates/proteins at 40 µg per lane.

        Predicted band size: 27 kDa



        12% SDS-PAGE gel.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab226994? Please let us know so that we can cite the reference in this datasheet.

      ab226994 has been referenced in 1 publication.

      • Wang C  et al. Long Noncoding RNA LINC01134 Promotes Hepatocellular Carcinoma Metastasis via Activating AKT1S1 and NF-?B Signaling. Front Cell Dev Biol 8:429 (2020). PubMed: 32656205

      Customer reviews and Q&As

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