Key features and details
- Rabbit polyclonal to PRAS40 (phospho T246)
- Suitable for: IHC-P, ELISA, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRAS40 (phospho T246) antibody
See all PRAS40 primary antibodies
DescriptionRabbit polyclonal to PRAS40 (phospho T246)
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human PRAS40 (phospho T246) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q96B36
- HEK293T, A431, and HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 - 20 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml.|
|WB||Use a concentration of 1.5 - 3 µg/ml. Predicted molecular weight: 27 kDa.|
|ICC/IF||Use a concentration of 10 - 20 µg/ml.|
FunctionSubunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Tissue specificityWidely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
modificationsPhosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
Cellular localizationCytoplasm > cytosol. Found in the cytosolic fraction of the brain.
- Information by UniProt
- 40 kDa proline rich AKT substrate antibody
- 40 kDa proline-rich AKT substrate antibody
- AKT1 S1 antibody
All lanes : Anti-PRAS40 (phospho T246) antibody (ab214100) at 1.5 µg/ml
Lane 1 : HEK293T cell extract
Lane 2 : A431 cell extract
Lane 3 : HeLa cell extract
Lane 4 : HEK293T cell extract with non-phospho-PRAS40 peptide
Lane 5 : HEK293T cell extract with immunizing phospho-PRAS40 (pThr246) peptide
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 27 kDa
Immunofluorescent analysis of HeLa cells labeling PRAS40 (phospho T246) with ab214100 at 10 μg/mL dilution, followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PRAS40 (phospho T246) with ab214100 at 10 μg/ml dilution followed by Goat Anti-Rabbit IgG-Biotin//ExtrAvidin®-Peroxidase.
ab214100 has been referenced in 1 publication.
- Li S et al. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3ß/ß-Catenin/cyclinD1 Signaling Pathway. Int J Biol Sci 13:976-984 (2017). PubMed: 28924379