Overview

  • Product name

    Anti-PRAS40 (phospho T246) antibody
    See all PRAS40 primary antibodies
  • Description

    Rabbit polyclonal to PRAS40 (phospho T246)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PRAS40 (phospho T246) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q96B36

  • Positive control

    • HEK293T, A431, and HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab214100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 20 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 1.5 - 3 µg/ml. Predicted molecular weight: 27 kDa.
ICC/IF Use a concentration of 10 - 20 µg/ml.

Target

  • Function

    Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  • Tissue specificity

    Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
  • Post-translational
    modifications

    Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • Cellular localization

    Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa proline rich AKT substrate antibody
    • 40 kDa proline-rich AKT substrate antibody
    • AKT1 S1 antibody
    • AKT1 substrate 1 (proline rich) antibody
    • AKT1 substrate 1 antibody
    • AKT1S 1 antibody
    • AKT1S1 antibody
    • AKTS1_HUMAN antibody
    • Lobe antibody
    • MGC2865 antibody
    • PRAS 40 antibody
    • PRAS antibody
    • PRAS40 antibody
    • Proline rich akt substrate antibody
    • Proline rich Akt substrate 40 kDa antibody
    • Proline-rich AKT1 substrate 1 antibody
    see all

Images

  • All lanes : Anti-PRAS40 (phospho T246) antibody (ab214100) at 1.5 µg/ml

    Lane 1 : HEK293T cell extract
    Lane 2 : A431 cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : HEK293T cell extract with non-phospho-PRAS40 peptide
    Lane 5 : HEK293T cell extract with immunizing phospho-PRAS40 (pThr246) peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 27 kDa

  • Immunofluorescent analysis of HeLa cells labeling PRAS40 (phospho T246) with ab214100 at 10 μg/mL dilution, followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PRAS40 (phospho T246) with ab214100 at 10 μg/ml dilution followed by Goat Anti-Rabbit IgG-Biotin//ExtrAvidin®-Peroxidase.

References

This product has been referenced in:

  • Li S  et al. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3ß/ß-Catenin/cyclinD1 Signaling Pathway. Int J Biol Sci 13:976-984 (2017). Read more (PubMed: 28924379) »
See 1 Publication for this product

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