Recombinant
RabMAb

Recombinant Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (ab134084)

Overview

  • Product name

    Anti-PRAS40 (phospho T246) antibody [EPR6262-107]
    See all PRAS40 primary antibodies
  • Description

    Rabbit monoclonal [EPR6262-107] to PRAS40 (phospho T246)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Threonine 246 of Human PRAS40.

  • Positive control

    • HeLa cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
    • Tissue specificity

      Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
    • Post-translational
      modifications

      Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
    • Cellular localization

      Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
    • Information by UniProt
    • Database links

    • Alternative names

      • 40 kDa proline rich AKT substrate antibody
      • 40 kDa proline-rich AKT substrate antibody
      • AKT1 S1 antibody
      • AKT1 substrate 1 (proline rich) antibody
      • AKT1 substrate 1 antibody
      • AKT1S 1 antibody
      • AKT1S1 antibody
      • AKTS1_HUMAN antibody
      • Lobe antibody
      • MGC2865 antibody
      • PRAS 40 antibody
      • PRAS antibody
      • PRAS40 antibody
      • Proline rich akt substrate antibody
      • Proline rich Akt substrate 40 kDa antibody
      • Proline-rich AKT1 substrate 1 antibody
      see all

    Images

    • All lanes : Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (ab134084) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa

    References

    ab134084 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134084.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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