Product nameAnti-PRAS40 (phospho T246) antibody [EPR6262-107]
See all PRAS40 primary antibodies
DescriptionRabbit monoclonal [EPR6262-107] to PRAS40 (phospho T246)
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide corresponding to residues surrounding Threonine 246 of Human PRAS40.
- HeLa cell lysates.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).|
|IP||1/10 - 1/100.|
FunctionSubunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Tissue specificityWidely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
modificationsPhosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
Cellular localizationCytoplasm > cytosol. Found in the cytosolic fraction of the brain.
- Information by UniProt
- 40 kDa proline rich AKT substrate antibody
- 40 kDa proline-rich AKT substrate antibody
- AKT1 S1 antibody
All lanes : Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (ab134084) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
ab134084 has not yet been referenced specifically in any publications.