Key features and details
- Mouse monoclonal [6G2] to PRC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-PRC1 antibody [6G2]
See all PRC1 primary antibodies
DescriptionMouse monoclonal [6G2] to PRC1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 1/150 of Human PRC1
- HeLa cell extract
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesab39069 is an affinity purified IgG.
Light chain typekappa
Our Abpromise guarantee covers the use of ab39069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa.|
FunctionCross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
Sequence similaritiesBelongs to the MAP65/ASE1 family.
DomainMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
modificationsPhosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
- Information by UniProt
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extracts
secondary conjugated to HRP
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
ab39069 has not yet been referenced specifically in any publications.