Key features and details
- Rabbit polyclonal to PRC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PRC1 antibody
See all PRC1 primary antibodies
DescriptionRabbit polyclonal to PRC1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A 19 amino acid synthetic peptide near the center of Human PRC1 (NP_003972).
- Human skeletal muscle tissue lysate; Mouse skeletal muscle tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab140033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 72 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionCross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
Sequence similaritiesBelongs to the MAP65/ASE1 family.
DomainMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
modificationsPhosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
- Information by UniProt
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
Immunohistochemical analysis of paraffin-embedded Mouse skeletal tissue, labelling PRC1 with ab140033 at 2.5 µg/ml.
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with ab140033 at 20 ug/mL.
Lane 1 : Anti-PRC1 antibody (ab140033) at 0.5 µg/ml
Lane 2 : Anti-PRC1 antibody (ab140033) at 1 µg/ml
All lanes : Human skeletal muscle tissue lysate
Predicted band size: 72 kDa
ab140033 has been referenced in 1 publication.
- Ting W et al. Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1. Mol Ther Nucleic Acids 21:428-440 (2020). PubMed: 32668390