RabMAb

Anti-PRC1 antibody [EP1513Y] (ab51248)

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Overview

  • Product name

    Anti-PRC1 antibody [EP1513Y]
    See all PRC1 primary antibodies

  • Description

    Rabbit monoclonal [EP1513Y] to PRC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PRC1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035), human cervical carcinoma or HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1513Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/10.

Target

  • Function

    Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.

  • Sequence similarities

    Belongs to the MAP65/ASE1 family.

  • Domain

    Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.

  • Post-translational
    modifications

    Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.

  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Anaphase spindle elongation 1 homolog antibody
    • ASE1 antibody
    • PRC1 antibody
    • PRC1_HUMAN antibody
    • Protein regulating cytokinesis 1 antibody
    • Protein regulator of cytokinesis 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRC1 antibody [EP1513Y] (ab51248)

    Ab51248 (1:100) staining human PRC1 in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Western blot - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Anti-PRC1 antibody [EP1513Y] (ab51248) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Immunofluorescent staining of HeLa cells using ab51248 (1:100).
  • Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Immunocytochemistry/ Immunofluorescence - Anti-PRC1 antibody [EP1513Y] (ab51248)This image is courtesy of an anonymous Abreview

    ab51248 staining PRC1 in human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked in 1% serum for 1 hour at 37°C without permeation step. The primary antibody was diluted 1/100 (PBS) and incubated with sample for 1 hour at 20°C. An Alexa Fluor® 488 conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used as secondary.

    See Abreview

  • Flow Cytometry - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Flow Cytometry - Anti-PRC1 antibody [EP1513Y] (ab51248)
    Overlay histogram showing HeLa cells stained with ab51248 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51248, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

  • Dwivedi D  et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
  • Yi L  et al. Long noncoding RNA PTPRG-AS1 acts as a microRNA-194-3p sponge to regulate radiosensitivity and metastasis of nasopharyngeal carcinoma cells via PRC1. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30993702) »
See all 7 Publications for this product

Publishing research using ab51248? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-PRC1 antibody [EP1513Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 18 2014

Western blot abreview for Anti-PRC1 antibody [EP1513Y]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Nov 18 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-PRC1 antibody [EP1513Y]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast Cancer)
Specification
Breast Cancer
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 30 2009

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PRC1 antibody [EP1513Y]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Throid Cancer)
Specification
Throid Cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer (pH6.0)
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 30 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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