Product nameAnti-PRC1 (phospho T481) antibody [EP1514Y]
See all PRC1 primary antibodies
DescriptionRabbit monoclonal [EP1514Y] to PRC1 (phospho T481)
Specificityab62366 detects PRC1 phosphorylated at Theonine 481.
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Dot blotmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1
- HeLa cells and HeLa whole cell lysate (ab150035).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab62366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Dot blot||Use at an assay dependent concentration.|
FunctionCross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
Sequence similaritiesBelongs to the MAP65/ASE1 family.
DomainMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
modificationsPhosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
- Information by UniProt
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution
Lane 1 : HeLa cell lysates (untreated)
Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
This product has been referenced in:
- Bartlett E et al. Interplay of Histone Marks with Serine ADP-Ribosylation. Cell Rep 24:3488-3502.e5 (2018). Read more (PubMed: 30257210) »
- Cundell MJ et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »