Recombinant Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
![Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)](https://www.abcam.com/ps/products/247/ab247354/Images/ab247354-2-anti-prc1-phospho-t481-antibody-ep1514y-bsa-and-azide-free-dot-blot.jpg "Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free
- Suitable for: ICC/IF, Dot blot, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free
See all PRC1 primary antibodies -
Description
Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects PRC1 phosphorylated at Theonine 481.
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Tested applications
Suitable for: ICC/IF, Dot blot, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PRC1 (phospho T481). The exact sequence is proprietary.
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General notes
ab247354 is the carrier-free version of ab62366 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1514Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab247354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. -
Sequence similarities
Belongs to the MAP65/ASE1 family. -
Domain
Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain. -
Post-translational
modificationsPhosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 9055 Human
- Omim: 603484 Human
- SwissProt: O43663 Human
- Unigene: 366401 Human
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Alternative names
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
see all
Images
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Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)This data was developed using ab62366, the same antibody clone in a different buffer formulation.Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
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![Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)](https://www.abcam.com/ps/products/247/ab247354/Images/ab247354-402292-anti-prc1-phospho-t481-antibody-ep1514y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution
Lane 1 : HeLa cell lysates (untreated)
Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDaThis data was developed using ab62366, the same antibody clone in a different buffer formulation.
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![Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)](https://www.abcam.com/ps/products/247/ab247354/Images/ab247354-3-benefits-of-recombinant-antibodies.png)
Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247354 has not yet been referenced specifically in any publications.
