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RecombinantRabMAb

Recombinant Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

  • Datasheet
  • Certificate of Compliance
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Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
  • Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
  • Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free
  • Suitable for: ICC/IF, Dot blot, WB, IHC-P
  • Reacts with: Human

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Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

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Overview

  • Product name

    Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free
    See all PRC1 primary antibodies
  • Description

    Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects PRC1 phosphorylated at Theonine 481.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PRC1 (phospho T481). The exact sequence is proprietary.

  • General notes

    ab247354 is the carrier-free version of ab62366 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP1514Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis

Associated products

  • Alternative Versions

    • Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)

Applications

Our Abpromise guarantee covers the use of ab247354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
    • Sequence similarities

      Belongs to the MAP65/ASE1 family.
    • Domain

      Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
    • Post-translational
      modifications

      Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
    • Target information above from: UniProt accession O43663 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9055 Human
      • Omim: 603484 Human
      • SwissProt: O43663 Human
      • Unigene: 366401 Human
      • Alternative names

        • Anaphase spindle elongation 1 homolog antibody
        • ASE1 antibody
        • PRC1 antibody
        • PRC1_HUMAN antibody
        • Protein regulating cytokinesis 1 antibody
        • Protein regulator of cytokinesis 1 antibody
        see all

      Images

      • Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
        Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
        This data was developed using ab62366, the same antibody clone in a different buffer formulation.Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
      • Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
        Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
        All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution

        Lane 1 : HeLa cell lysates (untreated)
        Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution

        Predicted band size: 72 kDa
        Observed band size: 72 kDa



        This data was developed using ab62366, the same antibody clone in a different buffer formulation.

      • Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
        Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247354? Please let us know so that we can cite the reference in this datasheet.

      ab247354 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab247354.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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