Recombinant
RabMAb

Recombinant Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

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Overview

  • Product name

    Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free
    See all PRC1 primary antibodies

  • Description

    Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects PRC1 phosphorylated at Theonine 481.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human PRC1 (phospho T481). The exact sequence is proprietary.

  • General notes

    ab247354 is the carrier-free version of ab62366 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP1514Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.

    • Sequence similarities

      Belongs to the MAP65/ASE1 family.

    • Domain

      Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.

    • Post-translational
      modifications

      Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.

    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
    • Information by UniProt

    • Database links

      • Alternative names

        • Anaphase spindle elongation 1 homolog antibody
        • ASE1 antibody
        • PRC1 antibody
        • PRC1_HUMAN antibody
        • Protein regulating cytokinesis 1 antibody
        • Protein regulator of cytokinesis 1 antibody
        see all

      References

      ab247354 has not yet been referenced specifically in any publications.

      Publishing research using ab247354? Please let us know so that we can cite the reference in this datasheet.

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