Anti-PRCP antibody (ab231526)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PRCP antibody
See all PRCP primary antibodies -
Description
Rabbit polyclonal to PRCP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse PRCP aa 169-443. (N-terminal His and GST Tag; Expressed in E.coli).
Sequence:QPVIAIGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGMVPCGEFMK IVTNDFRKSGPYCSESIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSE KIPTLKGWIAETWVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSD TVLLQNIFQALSVYYNYSGQAACLNISQTTTSSLGSMGWSFQACTEMVMP FCTNGIDDMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNISSHS NIIFSNGELDPWSGGGVTRDITDTL
Database link: Q7TMR0 -
Positive control
- IHC-P: Mouse kidney tissue. WB: Mouse kidney lysate; Recombinant mouse PRCP protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231526 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab231526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 56 kDa. |
Target
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Function
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. -
Tissue specificity
Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney. -
Sequence similarities
Belongs to the peptidase S28 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 72461 Mouse
- SwissProt: Q7TMR0 Mouse
- Unigene: 389969 Mouse
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Alternative names
- Angiotensinase C antibody
- HUMPCP antibody
- Lysosomal carboxypeptidase C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRCP antibody (ab231526)Formalin-fixed, paraffin-embedded mouse kidney tissue stained for PRCP using ab231526 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-PRCP antibody (ab231526)Anti-PRCP antibody (ab231526) at 2 µg/ml + Recombinant mouse PRCP protein
Predicted band size: 56 kDa -
Western blot - Anti-PRCP antibody (ab231526)Anti-PRCP antibody (ab231526) at 2 µg/ml + Mouse kidney lysate
Predicted band size: 56 kDa
Datasheets and documents
References
ab231526 has not yet been referenced specifically in any publications.
