Anti-PRCP antibody - N-terminal (ab171846)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PRCP antibody - N-terminal
See all PRCP primary antibodies -
DescriptionRabbit polyclonal to PRCP - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human PRCP aa 113-143 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P42785 -
Positive control
- MDA-MB231 and HeLa cell lysates, Human liver tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab171846 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab171846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 56 kDa. | |
| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionCleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
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Tissue specificityHighest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
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Sequence similaritiesBelongs to the peptidase S28 family.
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Cellular localizationLysosome.
- Information by UniProt
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Database links
- Entrez Gene: 5547 Human
- Omim: 176785 Human
- SwissProt: P42785 Human
- Unigene: 523936 Human
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Alternative names
- Angiotensinase C antibody
- HUMPCP antibody
- Lysosomal carboxypeptidase C antibody
see all
Images
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Western blot - Anti-PRCP antibody - N-terminal (ab171846)All lanes : Anti-PRCP antibody - N-terminal (ab171846) at 1/100 dilution
Lane 1 : MDA-MB231 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRCP antibody - N-terminal (ab171846)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling PRCP with ab171846 at 1/10 dilution. IHC was followed by peroxidase treatment.
Datasheets and documents
References
This product has been referenced in:
- Krochmal M et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). Read more (PubMed: 28831120) »
