Overview

  • Product name
    Anti-PRCP antibody - N-terminal
    See all PRCP primary antibodies
  • Description
    Rabbit polyclonal to PRCP - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PRCP aa 113-143 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P42785

  • Positive control
    • MDA-MB231 and HeLa cell lysates, Human liver tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171846 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 56 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
  • Tissue specificity
    Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
  • Sequence similarities
    Belongs to the peptidase S28 family.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Angiotensinase C antibody
    • HUMPCP antibody
    • Lysosomal carboxypeptidase C antibody
    • Lysosomal Pro X carboxypeptidase antibody
    • Lysosomal Pro-X carboxypeptidase antibody
    • MGC2202 antibody
    • PCP antibody
    • PCP_HUMAN antibody
    • PRCP antibody
    • Proline carboxypeptidase antibody
    • Prolylcarboxypeptidase (angiotensinase C) antibody
    • Prolylcarboxypeptidase antibody
    • prolylcarboxypeptidase isoform 1 preproprotein antibody
    see all

Images

  • All lanes : Anti-PRCP antibody - N-terminal (ab171846) at 1/100 dilution

    Lane 1 : MDA-MB231 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 56 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling PRCP with ab171846 at 1/10 dilution. IHC was followed by peroxidase treatment.

References

This product has been referenced in:
  • Krochmal M  et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). Read more (PubMed: 28831120) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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