Key features and details
- Rabbit polyclonal to PRD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRD antibody
See all PRD primary antibodies
DescriptionRabbit polyclonal to PRD
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Giant Panda
Synthetic peptide within Human PRD aa 443-493. The exact sequence is proprietary.
Database link: P12955
- WB: HeLa, HEK-293T and Jurkat whole cell lysates.
This product was previously labelled as PEPD
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226414 was affinity purified using an epitope specific to PRD immobilized on solid support.
Our Abpromise guarantee covers the use of ab226414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 55 kDa.|
FunctionSplits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
Involvement in diseaseDefects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait.
Sequence similaritiesBelongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.
- Information by UniProt
- Aminoacyl L proline hydrolase antibody
- Imidodipeptidase antibody
- MGC10905 antibody
All lanes : Anti-PRD antibody (ab226414) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 3 minutes
Cell lysates were prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226414 has not yet been referenced specifically in any publications.