Product nameAnti-PRD antibody [EPR16959]
See all PRD primary antibodies
DescriptionRabbit monoclonal [EPR16959] to PRD
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
within Human PRD aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P12955
- HepG2, SH-SY5Y, 293 whole cell lysates; Human fetal kidney and placenta lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab197890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
FunctionSplits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
Involvement in diseaseDefects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait.
Sequence similaritiesBelongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.
- Information by UniProt
- Aminoacyl L proline hydrolase antibody
- Imidodipeptidase antibody
- MGC10905 antibody
All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 1 minute
Blocking and dilution buffer: 5% NFDM/TBST
All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal placenta lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 30 seconds
Blocking/dilution buffer: 5% NFDM /TBST
ab197890 has not yet been referenced specifically in any publications.