Recombinant Anti-PRD antibody [EPR16959] (ab197890)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16959] to PRD
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PRD antibody [EPR16959]
See all PRD primary antibodies -
Description
Rabbit monoclonal [EPR16959] to PRD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
within Human PRD aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P12955 -
Positive control
- HepG2, SH-SY5Y, 293 whole cell lysates; Human fetal kidney and placenta lysates.
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General notes
This product was previously labelled as PEPD
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16959 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab197890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. -
Involvement in disease
Defects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. -
Sequence similarities
Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5184 Human
- Omim: 613230 Human
- SwissProt: P12955 Human
- Unigene: 36473 Human
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Alternative names
- Aminoacyl L proline hydrolase antibody
- Imidodipeptidase antibody
- MGC10905 antibody
see all
Images
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All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST
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All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 30 secondsBlocking/dilution buffer: 5% NFDM /TBST
Datasheets and documents
Certificate of Compliance
References (0)
ab197890 has not yet been referenced specifically in any publications.