Recombinant Anti-PRD antibody [EPR16959] (ab197890)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PRD antibody [EPR16959]
See all PRD primary antibodies -
Description
Rabbit monoclonal [EPR16959] to PRD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
within Human PRD aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P12955 -
Positive control
- HepG2, SH-SY5Y, 293 whole cell lysates; Human fetal kidney and placenta lysates.
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General notes
This product was previously labelled as PEPD
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16959 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab197890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. -
Involvement in disease
Defects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. -
Sequence similarities
Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5184 Human
- Omim: 613230 Human
- SwissProt: P12955 Human
- Unigene: 36473 Human
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Alternative names
- Aminoacyl L proline hydrolase antibody
- Imidodipeptidase antibody
- MGC10905 antibody
see all
Images
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![Western blot - Anti-PRD antibody [EPR16959] (ab197890)](https://www.abcam.com/ps/products/197/ab197890/Images/ab197890-240224-ab197890.jpg)
Western blot - Anti-PRD antibody [EPR16959] (ab197890)All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-PRD antibody [EPR16959] (ab197890)](https://www.abcam.com/ps/products/197/ab197890/Images/ab197890-240226-anti-pepd-antibody-epr16959-western-blot.jpg)
Western blot - Anti-PRD antibody [EPR16959] (ab197890)All lanes : Anti-PRD antibody [EPR16959] (ab197890) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Exposure time: 30 secondsBlocking/dilution buffer: 5% NFDM /TBST
Protocols
Datasheets and documents
References
ab197890 has not yet been referenced specifically in any publications.
