Product nameAnti-PRDM13 antibody
See all PRDM13 primary antibodies
DescriptionRabbit polyclonal to PRDM13
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
- Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
RelevancePRDM13 may be involved in transcriptional regulation.
- MU MB 20.220 antibody
- PFM 10 antibody
- PFM10 antibody
All lanes : Anti-PRDM13 antibody (ab66665) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Jurkat cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab66665 has not yet been referenced specifically in any publications.