Product nameAnti-PRDM14 antibody
See all PRDM14 primary antibodies
DescriptionRabbit polyclonal to PRDM14
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
- Jurkat cell lysate for Western blot, Human lung tissue for Immunohistochemistry (paraffin embedded).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab28638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
Contains 1 SET domain.
- Information by UniProt
- MGC59730 antibody
- PFM 11 antibody
- PFM11 antibody
Anti-PRDM14 antibody (ab28638) at 1.25 µg/ml + Jurkat cell lysate 10ug.
HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000
Predicted band size: 64 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
ab28638 staining PRDM14 in Human lung by Immunohistochemistry (FFPE-sections) at 4-8ug/ml. Positive label Alveolar cells (arrows).
This product has been referenced in:
- Wang H et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
- Nishikawa N et al. Gene amplification and overexpression of PRDM14 in breast cancers. Cancer Res 67:9649-57 (2007). Read more (PubMed: 17942894) »