Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [6D3] to PRDM1/Blimp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Product nameAnti-PRDM1/Blimp1 antibody [6D3]
See all PRDM1/Blimp1 primary antibodies
DescriptionRat monoclonal [6D3] to PRDM1/Blimp1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: NIH/3T3 and RAW 264.7 whole cell lysates. IHC-P: Mouse small intestine tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityIon Exchange Chromatography
Light chain typekappa
Our Abpromise guarantee covers the use of ab243146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.89 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 96 kDa).|
|IHC-P||Use a concentration of 17.8 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTranscriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain.
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
All lanes : Anti-PRDM1/Blimp1 antibody [6D3] (ab243146) at 0.89 µg/ml
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 96 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8µg/ml followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID: 21878906). Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243146 has been referenced in 1 publication.
- Kallies A et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med 200:967-77 (2004). PubMed: 15492122