Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRDM1/Blimp1 antibody
See all PRDM1/Blimp1 primary antibodies
DescriptionGoat polyclonal to PRDM1/Blimp1
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab106766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
1 hour primary incubation is recommended for this product.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionTranscriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain.
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
All lanes : Anti-PRDM1/Blimp1 antibody (ab106766) at 0.1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 nuclear lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab106766 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab106766 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Anti-PRDM1/Blimp1 antibody (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
ab106766 has been referenced in 1 publication.
- Kim SJ et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). ChIP ; Mouse . PubMed: 23298838