Anti-PRDM4/PFM1 antibody (ab126939)
Key features and details
- Rabbit polyclonal to PRDM4/PFM1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PRDM4/PFM1 antibody
See all PRDM4/PFM1 primary antibodies -
Description
Rabbit polyclonal to PRDM4/PFM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 559-758 of Human PRDM4/PFM1 (Q9UKN5).
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Positive control
- PC3 and U-87 MG whole cell lysate; Human HBL435 xenograft and Mouse Muscle tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May function as a transcription factor involved in cell differentiation. -
Tissue specificity
Expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate. -
Sequence similarities
Contains 6 C2H2-type zinc fingers.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11108 Human
- Entrez Gene: 72843 Mouse
- Omim: 605780 Human
- SwissProt: Q9UKN5 Human
- SwissProt: Q80V63 Mouse
- Unigene: 506655 Human
- Unigene: 25307 Mouse
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Alternative names
- MGC45046 antibody
- PFM 1 antibody
- PFM1 antibody
see all
Images
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All lanes : Anti-PRDM4/PFM1 antibody (ab126939) at 1/5000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa
7.5% SDS PAGE -
ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Human HBL435 xenograft tissue by Immunohistochemistry.
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ab126939, at 1/500 dilution, staining PRDM4/PFM1 in Paraffin-embedded Mouse Muscle tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126939 has been referenced in 1 publication.
- Yang WT et al. PRDM4 inhibits cell proliferation and tumorigenesis by inactivating the PI3K/AKT signaling pathway through targeting of PTEN in cervical carcinoma. Oncogene 40:3318-3330 (2021). PubMed: 33846573