Overview

  • Product name

    Anti-PRDM4/PFM1 antibody [EPR9432]
    See all PRDM4/PFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9432] to PRDM4/PFM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human PRDM4/PFM1 (Q9UKN5).

  • Positive control

    • HeLa, 293T, PC3 and SH SY5Y cell lysates; Human kidney tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.

    • All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293 cell lysate
      Lane 3 : PC-3 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 88 kDa
      Observed band size: 88 kDa



      Blocking buffer: 5% NFDM/TBST.

    • All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H + L) at 1/1000 dilution

      Predicted band size: 88 kDa
      Observed band size: 88 kDa



      Blocking buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.

    • Immunocytochemistry/Immunofluorescence analysis of 293 cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.

    References

    This product has been referenced in:

    • Song NJ  et al. PI3Ka-Akt1-mediated Prdm4 induction in adipose tissue increases energy expenditure, inhibits weight gain, and improves insulin resistance in diet-induced obese mice. Cell Death Dis 9:876 (2018). Read more (PubMed: 30158592) »
    • Rolev K  et al. Identification of Prdm genes in human corneal endothelium. Exp Eye Res 159:114-122 (2017). Read more (PubMed: 28228349) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Human Cell (Human corneal endothelial cells (HCEC-12))
    Specification
    Human corneal endothelial cells (HCEC-12)
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Mr. Kostadin Rolev

    Verified customer

    Submitted Apr 23 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 1 (pH 6) for 20 min Cat. No: AR9961
    Sample
    Human Tissue sections (Human corneas)
    Specification
    Human corneas
    Permeabilization
    Yes - Heat mediated at 100oC
    Fixative
    Formaldehyde

    Mr. Kostadin Rolev

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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