Overview

  • Product name

    Preadipocyte Isolation kit
  • Sample type

    Tissue
  • Species reactivity

    Reacts with: Mouse, Rat, Other species, Mammals
    Predicted to work with: Mammals
  • Product overview

    Preadipocyte Isolation kit (ab196988) provides the reagents and tools to isolate the stromal vascular fraction from adipose tissue. The resulting stromal vascular fraction is cultured on a tissue culture plate, to which the preadipocytes adhere. The preadipocytes retain the ability to proliferate and differentiate into adipocytes when treated with differentiation-inducing components. The preadipocytes can be used to study the process of adipogenesis, while the differentiated adipocytes can be used to study lipolysis, endocrine activity, cell-signaling and metabolic dysfunction.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 5 g
    Cell Strainer (100 μm) 10 units
    Cell Strainer (70 μm) 10 units
    Collagenase NM, blue 1 x 10ml
    Collagenase Stop Buffer NM 1 x 90ml
    PBS 1 x 90ml
    Red Blood Cell Lysis Buffer 1 x 10ml
  • Research areas

Images

  • Preadipocytes from mouse adipose tissue after 4 days of culturing.

  • Adipocytes 7 days after differentiation from preadipocytes using a 3T3-L1 Differentiation Kit. Visible lipid droplets confirm the isolation of preadipocytes.

Protocols

References

This product has been referenced in:

  • Singh SP  et al. Kavain Reduces Porphyromonas gingivalis-Induced Adipocyte Inflammation: Role of PGC-1a Signaling. J Immunol N/A:N/A (2018). Read more (PubMed: 30037847) »
See 1 Publication for this product

Customer reviews and Q&As

I tried many different products to get better mice primary adipocyte cells but failed then I bought abcam primary adipocyte cells isolation kit ab196988 to isolate primary adipocyte cells from visceral adipose tissue of mice which was knockout to PGC1 alpha, as recommended in their datasheets and I never had any problem with it. I got very beautiful adipocyte cells which I used for immunofluorescence with adiponectin antibody. I think its great kit to use and have everything in it. I think this is best product to isolate primary adipocytes from fat

Abcam user community

Verified customer

Submitted Aug 06 2018

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