Overview

  • Product name

    Anti-Prealbumin antibody [2E10C5]
    See all Prealbumin primary antibodies
  • Description

    Mouse monoclonal [2E10C5] to Prealbumin
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant full length protein corresponding to Human Prealbumin aa 1-147. Expressed in E coli.
    Sequence:

    MASHRLLLLC LAGLVFVSEA GPTGTGESKC PLMVKVLDAV RGSPAINVAV HVFRKAADDT WEPFASGKTS ESGELHGLTT EEEFVEGIYK VEIDTKSYWK ALGISPFHEH AEVVFTANDS GPRRYTIAAL LSPYSYSTTA VVTNPKE


    Database link: P02766

  • Positive control

    • Human Prealbumin (aa1-147) recombinant protein, Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate, A549 or MCF-7 cells, Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab204997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 16 kDa.

Target

  • Function

    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificity

    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in disease

    Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similarities

    Belongs to the transthyretin family.
  • Domain

    Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localization

    Secreted. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Images

  • All lanes : Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human liver cancer tissue labeling Prealbumin with ab204997 at a 1/200 dilution. DAB staining used.

  • Flow cytometric analysis of A549 cells labelling Prealbumin with ab204997 (green) at a 1/200 dilution and negative control (red).

  • Immunofluorescence analysis of MCF-7 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Immunofluorescence analysis of A549 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).

  • Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution + Human Prealbumin (aa1-147) recombinant protein

    Predicted band size: 16 kDa
    Additional bands at: 45.8 kDa. We are unsure as to the identity of these extra bands.

References

ab204997 has not yet been referenced specifically in any publications.

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