Key features and details
- Mouse monoclonal [2E10C5] to Prealbumin
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Prealbumin antibody [2E10C5]
See all Prealbumin primary antibodies
DescriptionMouse monoclonal [2E10C5] to Prealbumin
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human Prealbumin aa 1-147. Expressed in E coli.
MASHRLLLLC LAGLVFVSEA GPTGTGESKC PLMVKVLDAV RGSPAINVAV HVFRKAADDT WEPFASGKTS ESGELHGLTT EEEFVEGIYK VEIDTKSYWK ALGISPFHEH AEVVFTANDS GPRRYTIAAL LSPYSYSTTA VVTNPKE
Database link: P02766
- Human Prealbumin (aa1-147) recombinant protein, Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate, A549 or MCF-7 cells, Human liver cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204997 in the following tested applications.
|Flow Cyt||1/200 - 1/400.|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 16 kDa.|
FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
Sequence similaritiesBelongs to the transthyretin family.
DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
Cellular localizationSecreted. Cytoplasm.
- Information by UniProt
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
All lanes : Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human liver cancer tissue labeling Prealbumin with ab204997 at a 1/200 dilution. DAB staining used.
Flow cytometric analysis of A549 cells labelling Prealbumin with ab204997 (green) at a 1/200 dilution and negative control (red).
Immunofluorescence analysis of MCF-7 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).
Immunofluorescence analysis of A549 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).
Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution + Human Prealbumin (aa1-147) recombinant protein
Predicted band size: 16 kDa
Additional bands at: 45.8 kDa. We are unsure as to the identity of these extra bands.
ab204997 has not yet been referenced specifically in any publications.