Product nameAnti-Prealbumin antibody
See all Prealbumin primary antibodies
DescriptionSheep polyclonal to Prealbumin
Tested applicationsSuitable for: WB, IHC-P, Other, RID, Immunoelectrophoresis, Double Immunodiffusion, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
Human prealbumin purified from plasma.
- This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA
Concentration information loading...
Purification notesAntiserum is prepared by immunisation of sheep with Human Prealbumin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 15 kDa. PubMed: 16705188|
|IHC-P||1/1000. PubMed: 16705188|
|Other||Use at an assay dependent concentration.|
|RID||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|Double Immunodiffusion||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
Sequence similaritiesBelongs to the transthyretin family.
DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
Cellular localizationSecreted. Cytoplasm.
- Information by UniProt
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Matsuzaki T et al. Transthyretin deposition promotes progression of osteoarthritis. Aging Cell 16:1313-1322 (2017). IHC-P ; Mouse . Read more (PubMed: 28941045) »
- Moore GR et al. Complement and Humoral Adaptive Immunity in the Human Choroid Plexus: Roles for Stromal Concretions, Basement Membranes, and Epithelium. J Neuropathol Exp Neurol 75:415-28 (2016). Read more (PubMed: 26994633) »