Overview

  • Product name

    Anti-Prealbumin antibody [EPR20971]
    See all Prealbumin primary antibodies
  • Description

    Rabbit monoclonal [EPR20971] to Prealbumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein within Mouse Prealbumin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P07309

  • Positive control

    • WB: Mouse plasma and serum. IHC-P: Mouse cerebrum and liver tissues. IHC-Fr: Mouse brain (choroid plexus) and liver tissues. IP: Mouse plasma.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

We recommend to optimize primary antibody dilution depending on the tissue being tested.

WB 1/1000. Detects a band of approximately 15, 30 kDa (predicted molecular weight: 15 kDa).
IP 1/30.

Target

  • Function

    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificity

    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in disease

    Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similarities

    Belongs to the transthyretin family.
  • Domain

    Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localization

    Secreted. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Images

  • All lanes : Anti-Prealbumin antibody [EPR20971] (ab215202) at 1/1000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    Blocking/Dilution: 5% NFDM/TBST

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (choroid plexus) tissue labeling Prealbumin with ab215202 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the endothelial cells of choroid plexus, negative staining in the endothelial cells of blood vessels on mouse brain tissue section (PMID: 19539719; 20462362).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in choroid plexuses (arrows) of mouse cerebrum (PMID: 25758461; PMID: 19032346). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prealbumin with ab215202 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse liver (PMID: 25519307; PMID: 19032346). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Prealbumin with ab215202 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the hepatic cells of mouse liver tissue section (PMID: 25519307).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Prealbumin was immunoprecipitated from 0.35 mg of mouse plasma with ab215202 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215202 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution

    Lane 1: Mouse plasma 10 µg (Input). 

    Lane 2: ab215202 IP in mouse plasma. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215202 in mouse plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The band shown at 30 kDa is likely to be a dimer of Prealbumin based on the literature (PMID: 19279259).

References

ab215202 has not yet been referenced specifically in any publications.

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