Recombinant Anti-Prealbumin antibody [EPR2928(2)] (ab133284)
![Western blot - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)](https://www.abcam.com/ps/products/133/ab133284/Images/ab133284-146752-anti-prealbumin-antibody-epr29282-western-blot.jpg "Western blot - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2928(2)] to Prealbumin
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Prealbumin antibody [EPR2928(2)]
See all Prealbumin primary antibodies -
Description
Rabbit monoclonal [EPR2928(2)] to Prealbumin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Prealbumin aa 1-100. The exact sequence is proprietary.
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Positive control
- Human placenta, human heart, fetal kidney and fetal liver lysates; Human liver tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2928(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 15 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. -
Tissue specificity
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. -
Involvement in disease
Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. -
Sequence similarities
Belongs to the transthyretin family. -
Domain
Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. -
Cellular localization
Secreted. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7276 Human
- Omim: 176300 Human
- SwissProt: P02766 Human
- Unigene: 427202 Human
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Alternative names
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
see all
Images
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Western blot - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)All lanes : Anti-Prealbumin antibody [EPR2928(2)] (ab133284) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human heart lysate
Lane 3 : Fetal kidney lysate
Lane 4 : Fetal liver lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)](https://www.abcam.com/ps/products/133/ab133284/Images/ab133284-146751-anti-prealbumin-antibody-epr29282-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [EPR2928(2)] (ab133284)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Prealbumin with ab133284 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Prealbumin antibody [EPR2928(2)] (ab133284)](https://www.abcam.com/ps/products/133/ab133284/Images/ab133284-3-benefits-of-recombinant-antibodies.png)
Anti-Prealbumin antibody [EPR2928(2)] (ab133284)
Protocols
Datasheets and documents
References (0)
ab133284 has not yet been referenced specifically in any publications.
