Overview

  • Product name

    Anti-Prealbumin antibody [EPR3219]
    See all Prealbumin primary antibodies
  • Description

    Rabbit monoclonal [EPR3219] to Prealbumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Prealbumin aa 50-150.

  • Positive control

    • WB: Human plasma and placenta lysates IHC-P: Human liver and kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

HRP/AP polymerized antibody is recommended for use as a secondary antibody.

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
    • Tissue specificity

      Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
    • Involvement in disease

      Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
      Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
      Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
    • Sequence similarities

      Belongs to the transthyretin family.
    • Domain

      Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
    • Cellular localization

      Secreted. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amyloid polyneuropathy antibody
      • Amyloidosis I antibody
      • ATTR antibody
      • Carpal tunnel syndrome 1 antibody
      • CTS antibody
      • CTS1 antibody
      • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
      • Dystransthyretinemic hyperthyroxinemia antibody
      • Epididymis luminal protein 111 antibody
      • HEL111 antibody
      • HsT2651 antibody
      • PALB antibody
      • Prealbumin amyloidosis type I antibody
      • Prealbumin antibody
      • Prealbumin Thyroxine-binding antibody
      • Senile systemic amyloidosis antibody
      • TBPA antibody
      • Thyroxine binding prealbumin antibody
      • Transthyretin antibody
      • TTHY_HUMAN antibody
      • TTR antibody
      • TTR protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Prealbumin with purified ab92469 at 1/50 dilution (2.04 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-Prealbumin antibody [EPR3219] (ab92469) at 1/1000 dilution (Purified)

      Lane 1 : Human plasma lysates
      Lane 2 : Human placenta lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa

    • All lanes : Anti-Prealbumin antibody [EPR3219] (ab92469) at 1/1000 dilution (unpurified)

      Lane 1 : Human plasma lysate
      Lane 2 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 16 kDa

    • ab92469 (unpurified), at a 1/100 dilution, staining Prealbumin in paraffin embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Lu H  et al. iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy. Clin Proteomics 16:33 (2019). Read more (PubMed: 31384238) »
    • Minghui Z  et al. Analysis of Differentially Expressed Proteins Involved in Autoimmune Cirrhosis and Normal Serum by iTRAQ Proteomics. Proteomics Clin Appl N/A:e1700153 (2018). Read more (PubMed: 29999587) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015

    Answer

    Thank you for your inquiry.

    Unfortunately, ab75815, ab92469, and ab133284 (2434-1, 2524-1, 3168-1) were not raised from a region between a.a. 105 - 115. I took a look at the rest of the prealbumin antibodies in our catalogue and sadly none were raised from this region.

    I apologize that this information could not be more helpful. Please contact us with any other questions.

    Read More

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