Overview

  • Product name

    Precast Gel SDS-PAGE 10% (8x10cm,12well)
    See all SDS-PAGE Gel 10% (Triethanolamine) reagents
  • Description

    Precast Gel SDS-PAGE 10% (8x10cm,12well)
  • Tested applications

    Suitable for: SDS-PAGE, WBmore details
  • General notes

    This gel ab119206 is one of our Optiblot precast gels which offer superior rigidity and stability over traditional polyacrlyamide gels and are compatible with the most common tanks and systems. For convenience the precast gels come with the comb already removed as the cassette locks the fingers in place; for additional convenience there is no tape to be removed and cassettes can be opened by hand.

    Optiblot gels must be used with Optiblot buffers

    Learn more about Optiblot in our FAQs.

    Storage: For long term storage (up to 2 years) store at 4°C or for short-term storage (up to 6 months) store at room temperature.

    Number of Wells: 12

    This product was previously called Optiblot SDS Gel 10% (8 x 10cm) - 12 Well.


    Manufactured under patent number: US 5,769.22

Properties

Applications

Our Abpromise guarantee covers the use of ab119206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Images

  • Gel benefits - Click to expand



  • Migration Chart in kDa with SDS Buffer (ab119197)
  • Migration Chart in kDa with Reducing Buffer (ab119195) - Click to expand

Protocols

References

ab119206 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The 8X10 gels we produce are QC tested on the Biorad Mini-Protean system (so that we are duplicating what the customer sees). We have other Biorad compatible rigs, but we do the QC on the BR rig for just that reason.

The green gasket will have a notch which locks in over the plate from a biorad gel. This gasket needs to be reversed so the gasket is smooth all round and contacts with the gel plate evenly.

Perhaps there is some unknown issue occuring here preventing your customer for successfully using the Optiblot gels with the biorad gel rigs.

Read More

Answer

Thank you for contacting us.

I do not have firsthand experience with the Hoefer tank, but according to the product info on their website, they make gel rigs that will accept both the 8X10 and 10X10 size gels.

We have a wide range of WB reagents (buffers and stains: https://www.abcam.com/index.html?c=5644), electrophoresis gels, electrophoresis kits (https://www.abcam.com/index.html?c=5643) and protein ladders (https://www.abcam.com/index.html?c=5635) that may be of interest to your customer.

Product ab119210 is a prestained Marker that has been specifically formulated for use with Optiblot Gels and Buffers:

https://www.abcam.com/Optiblot-Prestained-Marker-ab119210.html

I hope this information is helpful to you. If you have any questions or if you need further help or a proforma quote then please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry about Optiblot pre-casted SDS gels. We recommend long term storage of up to 24 months store at 4°C or for 3 months at room temperature. For expiry date, please check the box of products.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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