Overview

  • Product name

    Precast Gel SDS-PAGE 4-20% (8x10cm,12well)
    See all SDS-PAGE Gel 4-20% (Triethanolamine) reagents
  • Description

    Precast Gel SDS-PAGE 4-20% (8x10cm,12well)
  • Tested applications

    Suitable for: WB, SDS-PAGEmore details
  • General notes

    This gel is one of our Optiblot precast gels which offer superior rigidity and stability over traditional polyacrlyamide gels and are compatible with the most common tanks and systems. For convenience the precast gels come with the comb already removed as the cassette locks the fingers in place; for additional convenience there is no tape to be removed and cassettes can be opened by hand.

    Optiblot gels must be used with Optiblot buffers.

    Learn more about Optiblot in our FAQs.

    Storage: For long term storage (up to 2 years) store at 4°C or for short-term storage (up to 6 months) store at room temperature.

    Number of Wells: 12

    This product was previously called Optiblot SDS Gel 4-20% (8x10cm) - 12 Well.


    Manufactured under patent number US 5,769.22

Properties

Applications

Our Abpromise guarantee covers the use of ab119209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Images

  • Gel benefits - Click to expand



  • Migration Chart in kDa with SDS Buffer (ab119197)
  • Migration Chart in kDa with Reducing Buffer (ab119195) - Click to expand

Protocols

References

ab119209 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.
The lab let me know that using Laemmli buffer is possible but far from ideal. The best results will be obtained with LDS sample buffer composition (ab119196).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answer

Thank you for your reply.
I'd suggest using the sample buffer that is compatible with the gel, namely ab119196.
https://www.abcam.com/optiblot-lds-sample-buffer-4x-ab119196.html
This is what is recommended to be used.
I can doublecheck this with the lab, but I don't think the standard Laemmli buffer would give you good results. I'll let you know.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.
Yes, the 8x10 Optiblot gels are compatible with the Bio-Rad Mini-PROTEAN tank.
Please also check our FAQ section on Optiblots:
https://www.abcam.com/index.html?pageconfig=resource&rid=14722
where your questions are being answered as well as other information is being provided:
5. Do I need to use Optiblot buffers?
Yes, Optiblot buffers must be used with Optiblot gels to achieve the best results. Other buffer systems will result in poor electrophoresis.
6. Can I make Optiblot buffers in my lab?
Yes, buffer recipes are available to download. (https://docs.abcam.com/pdf/protocols/Optiblot-Buffer-Recipes.pdf)
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I have a question about your Optiblot pre-casted SDS gel products. How long can I use those SDS gels after purchasing?

Thank you for your enquiry about Optiblot pre-casted SDS gels. We recommend long term storage of up to 24 months store at 4°C or for 3 months at room temperature. For expiry date, please check the box of products.

I hope this information helps but please do not hesitate to contact me for additional information or assistance.

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Answer

Thank you for attending our WB Webinar.

It is possible to cast your own gradient gels, but the process requires special equipment and is time consuming. If you would like to learn more about the protocol, see the following reference:

http://www.nationaldiagnostics.com/article_info.php/articles_id/54

We have recently added a range of Optiblot western blotting reagents to our catalog including several different precastgels:

ab119204 - Optiblot SDS Gel 4-8% (10 x 10cm)

ab119205 - Optiblot SDS Gel 4-20% (10 x 10cm)

ab119208 - Optiblot SDS Gel 4-8% (8 x 10cm)

ab119209 - Optiblot SDS Gel 4-20% (8x10cm)

I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for contacting Abcam and we appreciate your interest in our Optiblot product range. Although not specified on our website, I believe that the Optiblot SDS Gels, 4-20% (8x10cm) have 12 wells and can hold a maximum of 35ul per well. I am in the process of confirming this with my colleagues and will contact you when I find out for certain. Please let me know if can be of any further assistance to you in this, or any other matter.

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