Product namePrecast Gel SDS-PAGE 8% (8x10cm,17well)
See all SDS-PAGE Gel 8% (Triethanolamine) reagents
DescriptionPrecast Gel SDS-PAGE 8% (8x10cm,17well)
Tested applicationsSuitable for: WB, SDS-PAGEmore details
This gel is one of our Optiblot precast gels which offer superior rigidity and stability over traditional polyacrlyamide gels and are compatible with the most common tanks and systems. For convenience the precast gels come with the comb already removed as the cassette locks the fingers in place, for additional convenience there is no tape to be removed and cassettes can be opened by hand.
Learn more about Optiblot in our FAQs.
Optiblot gels must be used with Optiblot buffers
Storage: For long term storage (up to 2 years) store at 4°C or for short-term storage (up to 6 months) store at room temperature.
Number of Wells: 17
This product was previously called Optiblot SDS Gel 8% (8 x 10cm) - 17 Well.
Manufactured under patent number US 5,769.22
Storage instructionsPlease see notes section.
Storage bufferConstituents: 0.2% SDS, 5% Triethanolamine, 8% Polyacrylamide, 0.02% Acrylamide, 0.002% Bis-acrylamide
Concentration information loading...
Our Abpromise guarantee covers the use of ab139604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|SDS-PAGE||Use at an assay dependent concentration.|
Gel benefits - Click to expand
Migration Chart in kDa with Reducing Buffer (ab119195) - Click to expand
Migration Chart in kDa with SDS Buffer (ab119197) - Click to expand
SDS-PAGE using ab139604 - Optiblot SDS Gel 8% (8 x 10cm) - 17 Well (ab139604). Protein was extracted using RIPA buffer extraction, followed by treatment at 37°C for 30 minutes in Optiblot LDS Sample Buffer (4X). 100µg was loaded per well and run for 45 minutes at 180V. An Optiblot Low Fluorescence membrane was used for blotting in a tank system and run at 50V for 2 hours and 30 minutes using Optiblot TGS Blot Buffer. The membrane as washed in TBST twice (20 seconds each), methanol for 10 seconds, left to dry for 5 seconds before being stained with Ponceau-S for 2 minutes and washed with TBST for 2 minutes to visualise bands. Lane 1: Broad range molecular weight marker. Lane 2-17: 100µg whole cell lysate.
ab139604 has not yet been referenced specifically in any publications.