Overview

  • Product name

    Precast Gel SDS-PAGE 8% (8x10cm,17well)
    See all SDS-PAGE Gel 8% (Triethanolamine) reagents
  • Description

    Precast Gel SDS-PAGE 8% (8x10cm,17well)
  • Tested applications

    Suitable for: WB, SDS-PAGEmore details
  • General notes

    This gel is one of our Optiblot precast gels which offer superior rigidity and stability over traditional polyacrlyamide gels and are compatible with the most common tanks and systems. For convenience the precast gels come with the comb already removed as the cassette locks the fingers in place, for additional convenience there is no tape to be removed and cassettes can be opened by hand.

    Learn more about Optiblot in our FAQs.

    Optiblot gels must be used with Optiblot buffers

    Storage: For long term storage (up to 2 years) store at 4°C or for short-term storage (up to 6 months) store at room temperature.

    Number of Wells: 17

    This product was previously called Optiblot SDS Gel 8% (8 x 10cm) - 17 Well.

    Manufactured under patent number US 5,769.22

Properties

Applications

Our Abpromise guarantee covers the use of ab139604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Images

  • Gel benefits - Click to expand



  • Migration Chart in kDa with Reducing Buffer (ab119195) - Click to expand

  • Migration Chart in kDa with SDS Buffer (ab119197) - Click to expand

  • SDS-PAGE using ab139604 - Optiblot SDS Gel 8% (8 x 10cm) - 17 Well (ab139604). Protein was extracted using RIPA buffer extraction, followed by treatment at 37°C for 30 minutes in Optiblot LDS Sample Buffer (4X). 100µg was loaded per well and run for 45 minutes at 180V. An Optiblot Low Fluorescence membrane was used for blotting in a tank system and run at 50V for 2 hours and 30 minutes using Optiblot TGS Blot Buffer. The membrane as washed in TBST twice (20 seconds each), methanol for 10 seconds, left to dry for 5 seconds before being stained with Ponceau-S for 2 minutes and washed with TBST for 2 minutes to visualise bands. Lane 1: Broad range molecular weight marker. Lane 2-17: 100µg whole cell lysate.

Protocols

References

ab139604 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The Optiblot gel has exceeded our expectations!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The Optiblot SDS 17-well gel has exceeded our expectations when trying to identify a membrane bound protein (75 kDa) from whole cell lysate. The gels are very easy to handle and VERY robust and have not teared on us during use, ideal for my PhD student who was starting out with SDS-Page/Western Blots and had problems using thinner gels from other companies. The 17-well format, although accommodating small well-volumes (25uL) are ideal for rapid throughput of protein samples. When coupled with the Optiblot 4X loading buffer, and consistently good transfer onto Optiblot membranes I would highly recommend the Optiblot range, particularly those wishing to use a thicker/stronger membrane for running multiples samples.

Image legend/experimental summary:
Lane 1: Broad range molecular weight marker
Lane 2-17: 100ug whole cell lysate

Protein was extracted using RIPA buffer extraction, followed by treatment at 37 degrees Celsius for 30 minutes in Optiblot LDS Sample Buffer (4X). 100ug was loaded per well and run for 45 minutes at 180V. An Optiblot Low Fluorescence membrane was used for blotting in a tank system and run at 5V for 2 hours and 30 minutes using Optiblot TGS Blot Buffer. The membrane as washed in TBST twice (20 seconds each), methanol for 10 seconds, left to dry for 5 seconds before being stained with Ponceau-S for 2 minutes and washed with TBST for 2-minutes to visualise bands.

Dr. Raj Badhan

Verified customer

Submitted Jan 09 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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