Product nameAnti-PREP1 antibody
DescriptionRabbit polyclonal to PREP1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human PREP1 aa 201-436.
Database link: P55347
- WB: HepG2 nuclear lysate; IP: HeLa cell lysate; ICC/IF: HeLa cell lysate;
General notesKeep as concentrated solution.
This product was previously labelled as PKNOX1.1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/1000.|
Tissue specificityUbiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow.
Sequence similaritiesBelongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeobox protein PKNOX1 antibody
- Homeobox protein PREP-1 antibody
- Human homeobox containing protein antibody
All lanes : Anti-PREP1 antibody (ab154587) at 1/2000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE
HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. They were stained using ab154587 diluted at 1/1000 (green).
Nuclear staining Hoechst 33342 staining (blue).
HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of PREP1 protein by 2 μg of ab154587
Western Blot carried out by ab154587 at a 1/1000 dilution.
ab154587 has not yet been referenced specifically in any publications.