• Product name

    Anti-Presenilin 1/PS-1 antibody
    See all Presenilin 1/PS-1 primary antibodies
  • Description

    Rabbit polyclonal to Presenilin 1/PS-1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Presenilin 1/PS-1 aa 298-367 (C terminal).

  • General notes

    ab12272 is targeted against the C-terminal fragment, loop domain of Presenilin 1 and reacts with full-length and C-terminal fragments of presenilin 1 from human, monkey, mouse and hamster.



Our Abpromise guarantee covers the use of ab12272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.

Predicted molecular weight: 52kDa - This is the MW of the unprocessed precursor.

ICC 1/100 - 1/500.


  • Function

    Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • Tissue specificity

    Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • Involvement in disease

    Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • Sequence similarities

    Belongs to the peptidase A22A family.
  • Domain

    The PAL motif is required for normal active site conformation.
  • Post-translational

    Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Information by UniProt
  • Database links

  • Alternative names

    • AD3 antibody
    • Ad3h antibody
    • FAD antibody
    • Homo Sapiens Clone CC44 Senilin 1 antibody
    • Presenilin-1 CTF12 antibody
    • Protein S182 antibody
    • PS 1 antibody
    • PS-1 antibody
    • PS1-CTF12 antibody
    • PSEN1 antibody
    • PSN1_HUMAN antibody
    • PSNL1 antibody
    • S182 antibody
    see all


  • ICC/IF image of ab12272 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12272, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Filipovic A  et al. Biological and clinical implications of nicastrin expression in invasive breast cancer. Breast Cancer Res Treat 125:43-53 (2011). WB ; Human . Read more (PubMed: 20224929) »
See 1 Publication for this product

Customer reviews and Q&As


Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unseren Antikörpern hatten.

Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge zu Veränderung Ihres Protokolls machen:


Da ab12272 das rekombinante Protein erkennt und die richtige Bande auch in den Rattenproben zu sehen ist, handelt es sich hier um ein Hintergrundproblem in den Rattenproben.

1.) Ich schlage vor den Antikörper über Nacht bei 4C zu inkubieren. das wird die Spezifität erhöhen.

2.) Hintergrundbanden werden oft vom sekundären Antikörper verursacht. Darüber wird eine "no primary" Kontrolle Aufschluss geben.

3.) Presenilin 1 ist ein "multi-pass" Membranprotein, dass bei hoher Erhitzung und der Verwendung von Mercaptoethanol zum aggregieren neigt. Ich schlage deshalb vor die Proben mit DTT zu reduzieren und nur auf 70C fuer 15 Minuten zu erhitzen.

4.) Manche Antikörper produzieren aus ungeklärten Gründen bessere Ergebnisse, wenn BSA als Blockierungsreagenz verwendet wird.


ab75880 ist ein monoklonaler Antikörper aus dem Kaninchen.

1.) Ich schlage deshalb vor einen passenden sekundären Antikörper zu verwenden.Zum Beispiel denanti Rabbit sekundären Antikörper, der für ab12272 verwendet wurde.

2.) Dieser Antikörper ist von uns getestet, dass er Rattenproteinnicht erkennt, wie auf dem Datenblatt beschrieben: "does not react with rat and mouse". Ich schlage deshalb vor eine humane Probe zu testen. Ist das rekombinante Protein der Sequenz der Ratte angelehnt?

Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte und zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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