Overview

  • Product name

    Anti-Presenilin 1/PS-1 antibody
    See all Presenilin 1/PS-1 primary antibodies
  • Description

    Rabbit polyclonal to Presenilin 1/PS-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Presenilin 1/PS-1 (internal sequence). The exact sequence is proprietary.
    Database link: P49768

  • Positive control

    • IHC-P: Rat lung and adrenal gland tissues; SW480 and U373 xenograft tissues; mouse brain tissue. WB: Raji and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab227070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • Tissue specificity

    Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • Involvement in disease

    Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • Sequence similarities

    Belongs to the peptidase A22A family.
  • Domain

    The PAL motif is required for normal active site conformation.
  • Post-translational
    modifications

    Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Information by UniProt
  • Database links

  • Alternative names

    • AD3 antibody
    • Ad3h antibody
    • FAD antibody
    • Homo Sapiens Clone CC44 Senilin 1 antibody
    • Presenilin-1 CTF12 antibody
    • Protein S182 antibody
    • PS 1 antibody
    • PS-1 antibody
    • PS1-CTF12 antibody
    • PSEN1 antibody
    • PSN1_HUMAN antibody
    • PSNL1 antibody
    • S182 antibody
    see all

Images

  • Paraffin-embedded rat lung tissue stained for Presenilin 1/PS-1 using ab227070 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat adrenal gland tissue stained for Presenilin 1/PS-1 using ab227070 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded SW480 xenograft tissue stained for Presenilin 1/PS-1 using ab227070 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded U373 xenograft tissue stained for Presenilin 1/PS-1 using ab227070 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for Presenilin 1/PS-1 using ab227070 at 1/200 dilution in immunohistochemical analysis.

  • Anti-Presenilin 1/PS-1 antibody (ab227070) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS-PAGE gel.

  • Anti-Presenilin 1/PS-1 antibody (ab227070) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS-PAGE gel.

References

ab227070 has not yet been referenced specifically in any publications.

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