Overview

  • Product name

    Anti-Presenilin 1/PS-1 antibody [EP2000Y]
    See all Presenilin 1/PS-1 primary antibodies
  • Description

    Rabbit monoclonal [EP2000Y] to Presenilin 1/PS-1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Presenilin 1/PS-1 aa 300-400. The exact sequence is proprietary.
    Database link: P49768

  • Positive control

    • WB: HeLa, Mouse brain, Rat brain cell, Human fetal brain lysate. IHC-P: Human liver and Human glioma tissue. ICC/IF: A431 cells. Flow Cyt: HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab76083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 53 kDa.

For unpurified use at 1/5000 - 1/10000.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
    • Tissue specificity

      Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
    • Involvement in disease

      Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
      Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
    • Sequence similarities

      Belongs to the peptidase A22A family.
    • Domain

      The PAL motif is required for normal active site conformation.
    • Post-translational
      modifications

      Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
      After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
    • Information by UniProt
    • Database links

    • Alternative names

      • AD3 antibody
      • Ad3h antibody
      • FAD antibody
      • Homo Sapiens Clone CC44 Senilin 1 antibody
      • Presenilin-1 CTF12 antibody
      • Protein S182 antibody
      • PS 1 antibody
      • PS-1 antibody
      • PS1-CTF12 antibody
      • PSEN1 antibody
      • PSN1_HUMAN antibody
      • PSNL1 antibody
      • S182 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Presenilin 1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HEK293 whole cell lysate (20 µg)
      Lane 4: HeLa whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab76083 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab76083 was shown to recognize Presenilin 1 in wild-type HAP1 cells as signal was lost at the expected MW in Presenilin 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Presenilin 1 knockout samples were subjected to SDS-PAGE. Ab76083 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Presenilin 1/PS-1 with Purified ab76083 at 1:100 dilution (1.31 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 53 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?



      The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929. But we are not sure how to define the extra band around 75 KD. 

    • Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution (Purified) + Human fetal brain lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?



      The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929.

    • Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled antibody at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Immunohistochemical staining of paraffin-embedded human liver, using ab76083, at 1/100-1/250 dilution.

    References

    This product has been referenced in:

    • Bannai T  et al. Chronic cerebral hypoperfusion shifts the equilibrium of amyloid ß oligomers to aggregation-prone species with higher molecular weight. Sci Rep 9:2827 (2019). Read more (PubMed: 30808940) »
    • Al-Atrache Z  et al. Astrocytes infected with Chlamydia pneumoniae demonstrate altered expression and activity of secretases involved in the generation of ß-amyloid found in Alzheimer disease. BMC Neurosci 20:6 (2019). Read more (PubMed: 30786875) »
    See all 19 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Question
    Answer

    Thanks for your enquiry. Here is the pubmed link: http://www.ncbi.nlm.nih.gov/pubmed/10098862 I hope this is helpful. Please contact me again if you have any further questions.

    Read More
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (WT AND KO MEF)
    Loading amount
    30 µg
    Specification
    WT AND KO MEF
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Nov 08 2010

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa)
    Total protein in input
    200 µg
    Immuno-precipitation step
    Protein G
    Specification
    HeLa

    Abcam user community

    Verified customer

    Submitted Sep 22 2010

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HELA)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HELA
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Aug 30 2010

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton-x 0.5%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Aug 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up