Key features and details
- Rabbit polyclonal to Presenilin 2/AD5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Presenilin 2/AD5 antibody
See all Presenilin 2/AD5 primary antibodies
DescriptionRabbit polyclonal to Presenilin 2/AD5
Specificityab106351 does not cross react with other proteins.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
- Rat brain tissue lysate; MCF7, HeLa, SMMC, CEM whole cell lysates; Rat brain tissue.
Previously labelled as Presenilin 2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the peptidase A22A family.
DomainThe PAL motif is required for normal active site conformation.
modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
Phosphorylated on serine residues.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
- AD3L antibody
- AD3LP antibody
- AD4 antibody
Immunohistochemical analysis of paraffin-embedded Rat Kidney tissue labeling Presenilin 2 / AD5 with ab106351.
Immunohistochemical analysis of paraffin-embedded Rat Cardiac Muscle tissue labeling Presenilin 2 / AD5 with ab106351.
Immunohistochemical analysis of paraffin-embedded Human Mammary Cancer tissue labeling Presenilin 2 / AD5 with ab106351.
All lanes : Anti-Presenilin 2/AD5 antibody (ab106351) at 1 µg/ml
Lanes 1-2 : Rat brain tissue lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : SMMC whole cell lysate
Lane 6 : CEM whole cell lysate
Predicted band size: 50 kDa
ab106351 has been referenced in 1 publication.
- Xu TH et al. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to ?-secretase cleavage and the Aß42/Aß40 ratio. Cell Discov 2:16026 (2016). WB ; Human . PubMed: 27625790