Product nameAnti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y]
See all Presenilin 2/AD5 primary antibodies
DescriptionRabbit monoclonal [EP2613Y] to Presenilin 2/AD5 (phospho S327)
ab76114 detects Presenilin 2/AD5 phosphorylated on serine 327.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Presenilin 2/AD5.
- Hela cell lysates.
Previously labelled as Presenilin 2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76114 in the following tested applications.
|WB||1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).|
FunctionProbable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
Tissue specificityIsoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
Involvement in diseaseDefects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the peptidase A22A family.
DomainThe PAL motif is required for normal active site conformation.
modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
Phosphorylated on serine residues.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
- AD3L antibody
- AD3LP antibody
- AD4 antibody
All lanes : Anti-Presenilin 2/AD5 (phospho S327) antibody [EP2613Y] (ab76114) at 1/5000 dilution
Lane 1 : HeLa cell lysates un-treated
Lane 2 : HeLa cell lysates treated with AP
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
ab76114 has not yet been referenced specifically in any publications.