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RabMAb

Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)

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Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)

    Key features and details

    • Rabbit monoclonal [EP2614] to Presenilin 2/AD5 (phospho S330)
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614]
      See all Presenilin 2/AD5 primary antibodies
    • Description

      Rabbit monoclonal [EP2614] to Presenilin 2/AD5 (phospho S330)
    • Host species

      Rabbit
    • Specificity

      This antibody only detects Presenilin 2/AD5 when phosphorylated on Serine 330.

    • Tested applications

      Suitable for: WBmore details
      Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Synthetic peptide corresponding to Human Presenilin 2/AD5.

    • Positive control

      • HeLa cell lysates.
    • General notes

      Previously labelled as Presenilin 2. 

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20ºC.
    • Storage buffer

      pH: 7.20
      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      EP2614
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Notch Pathway
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Proteases
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Neurodegenerative disease
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Positive Controls

      • HeLa membrane extract lysate (ab29547)

    Applications

    Our Abpromise guarantee covers the use of ab109456 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
    • Tissue specificity

      Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
    • Involvement in disease

      Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the peptidase A22A family.
    • Domain

      The PAL motif is required for normal active site conformation.
    • Post-translational
      modifications

      Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
      Phosphorylated on serine residues.
    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Target information above from: UniProt accession P49810 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5664 Human
      • Entrez Gene: 19165 Mouse
      • Entrez Gene: 81751 Rat
      • Omim: 600759 Human
      • SwissProt: P49810 Human
      • SwissProt: Q61144 Mouse
      • SwissProt: O88777 Rat
      • Unigene: 25363 Human
      • Unigene: 330850 Mouse
      • Unigene: 11045 Rat
      see all
    • Alternative names

      • AD3L antibody
      • AD3LP antibody
      • AD4 antibody
      • AD5 antibody
      • Alzheimer disease 4 antibody
      • CMD1V antibody
      • E5-1 antibody
      • OTTHUMP00000035671 antibody
      • OTTHUMP00000035672 antibody
      • OTTHUMP00000228286 antibody
      • OTTHUMP00000228288 antibody
      • Presenilin 2 (Alzheimer disease 4) antibody
      • Presenilin 2 antibody
      • Presenilin-2 CTF subunit antibody
      • PS-2 antibody
      • PS2 antibody
      • Psen2 antibody
      • PSN2_HUMAN antibody
      • PSNL2 antibody
      • STM-2 antibody
      • STM2 antibody
      see all

    Images

    • Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)
      Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)
      All lanes : Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456) at 1/5000 dilution

      Lane 1 : HeLa cell lysate untreated with with Alkaline Phosphatase
      Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab109456? Please let us know so that we can cite the reference in this datasheet.

    ab109456 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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