Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)
![Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)](https://www.abcam.com/ps/products/109/ab109456/Images/ab109456-122333-anti-presenilin-2-ad5-phospho-s330-antibody-ep2614-western-blot.jpg "Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)")
Key features and details
- Rabbit monoclonal [EP2614] to Presenilin 2/AD5 (phospho S330)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614]
See all Presenilin 2/AD5 primary antibodies -
Description
Rabbit monoclonal [EP2614] to Presenilin 2/AD5 (phospho S330) -
Host species
Rabbit -
Specificity
This antibody only detects Presenilin 2/AD5 when phosphorylated on Serine 330.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Presenilin 2/AD5.
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Positive control
- HeLa cell lysates.
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General notes
Previously labelled as Presenilin 2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2614 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
Target
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Function
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. -
Tissue specificity
Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney. -
Involvement in disease
Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the peptidase A22A family. -
Domain
The PAL motif is required for normal active site conformation. -
Post-translational
modificationsHeterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
Phosphorylated on serine residues. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 5664 Human
- Entrez Gene: 19165 Mouse
- Entrez Gene: 81751 Rat
- Omim: 600759 Human
- SwissProt: P49810 Human
- SwissProt: Q61144 Mouse
- SwissProt: O88777 Rat
- Unigene: 25363 Human
see all -
Alternative names
- AD3L antibody
- AD3LP antibody
- AD4 antibody
see all
Images
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Western blot - Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456)All lanes : Anti-Presenilin 2/AD5 (phospho S330) antibody [EP2614] (ab109456) at 1/5000 dilution
Lane 1 : HeLa cell lysate untreated with with Alkaline Phosphatase
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Datasheets and documents
References (0)
ab109456 has not yet been referenced specifically in any publications.
