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    prestained-protein-ladder--mid-range-molecular-weight-10-180-kda-ab116027.pdf

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Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (5)

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SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
  • Western blot - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)

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Overview

  • Product name

    Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa)
    See all Prestained Protein Ladder reagents
  • Tested applications

    Suitable for: WB, SDS-PAGEmore details
  • General notes

    Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

    This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).

    This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Do not heat, dilute, add reducing agent before loading.

    Key product features:

    • Broad range: 10-180 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~2 - 3 µl for western blots

     Review other protein ladders in the unstained and prestained protein ladder guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    pH: 7.50
    Constituents: 0.02% Urea, 2% Sodium lauryl sulfate, 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 15% Glycerol (glycerin, glycerine), 1% Tris phosphate
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein ladders
  • Alternative names

    • Molecular weight marker
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder
    see all

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab116027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel.

SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
Notes
WB
Use at an assay dependent concentration.

~2 - 3 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel.

SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

Images

  • SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
    SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
    Gel 1: Tris-Glycine (~4-20%) Gel 2: Bis-Tris (12%) MOPS buffer Gel 3: Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
  • Western blot - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)
    Western blot - Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)This image is courtesy of an anonymous Abreview.

    Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5µl per well.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.

ab116027 has been referenced in 5 publications.

  • Burkitbayev M  et al. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Saudi J Biol Sci 28:891-900 (2021). PubMed: 33424381
  • Nikooie R  et al. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. J Cell Commun Signal 15:107-123 (2021). PubMed: 33398722
  • Shadman Z  et al. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. BMC Infect Dis 21:300 (2021). PubMed: 33761869
  • Bielecka ZF  et al. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Cell Biosci 7:71 (2017). PubMed: 29270287
  • Fiserova J  et al. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. J Cell Sci 127:124-36 (2014). PubMed: 24144701

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

abreview for Prestained Protein Ladder (Mid-range molecular weight)

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Perfect product!
The Prestained protein ladder (mid-range molecular weight) a ready to use three-color (red, green, blue) protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. Each vial contains 500 µl volume, sufficient for 200-500 gels. Figure: 1 µl per lane in SDS-PAGE gel and PVDF membrane.

Abcam user community

Verified customer

Submitted Nov 26 2021

Excellent protein marker for immunoblots

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5ul per well.

Abcam user community

Verified customer

Submitted Jan 29 2015

Question

Are these his tagged proteins?

Read More

Abcam community

Verified customer

Asked on Sep 07 2012

Answer

Merci pour votre appel.

Je peux vous confirmer que nos marqueurs de poids moléculaires, ab116027, ab116028 ou ab116029 ne contiennent aucune protéine avecun His tag.

Je tiens aussi à vous signaler, que des fois, en utilisant trop d'anticorps primaire, des protéines sont marquées de manière non-spécifique. Dans un cas comme ça, il suffit des fois de diluer l'anticorps primaire un peu plus.

J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Abcam Scientific Support

Answered on Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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