Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
Overview
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Product name
Prestained Protein Ladder – Broad molecular weight (10-245 kDa)
See all Prestained Protein Ladder reagents -
Description
Prestained Protein Ladder - Broad molecular weight (10-245 kDa) -
Tested applications
Suitable for: SDS-PAGE, WBmore details -
General notes
Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).
This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.
Key product features:
- Broad range: 10-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 - 2.5µl
Review other protein ladders in the unstained and prestained protein ladder guide.
This product was previously called Prism Ultra Protein Ladder (10-245 kDa).
Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Properties
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Form
Liquid -
Storage instructions
Please see notes section. -
Storage buffer
pH: 7.5
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| SDS-PAGE |
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis
on 15-well or 10-well mini-gel, respectively.
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| WB |
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
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| Notes |
|---|
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SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. |
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WB
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel. |
Images
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SDS-PAGE - Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab116028 has been referenced in 16 publications.
- Thejer BM et al. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. BMC Mol Cell Biol 21:24 (2020). PubMed: 32245408
- Depoix CL et al. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. Invest New Drugs 38:547-557 (2020). PubMed: 31155684
- Negri S et al. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Cell Mol Life Sci 77:2235-2253 (2020). PubMed: 31473770
- Chowdhury P et al. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Infect Genet Evol 85:104418 (2020). PubMed: 32561295
- Ghoshal S et al. Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Malar J 19:367 (2020). PubMed: 33054833