• Product name
    Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa)
    See all Prestained Protein Ladder reagents
  • Description
    Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)
  • Tested applications
    Suitable for: WB, SDS-PAGEmore details
  • General notes

    Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

    This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).

    This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

    Key product features:

    • Broad range: 3.5-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

    Review other protein ladders in the unstained and prestained protein ladder guide.


    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.


  • Form
  • Storage instructions
    Please see notes section.
  • Storage buffer
    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% SDS, 0.02% DTT, 15% Glycerol
  • Concentration information loading...
  • Research areas
  • Alternative names
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder


Our Abpromise guarantee covers the use of ab116029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.


  • Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.


ab116029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Merci pour votre appel.

Je peux vous confirmer que nos marqueurs de poids moléculaires, ab116027, ab116028 ou ab116029 ne contiennent aucune protéine avecun His tag.

Je tiens aussi à vous signaler, que des fois, en utilisant trop d'anticorps primaire, des protéines sont marquées de manière non-spécifique. Dans un cas comme ça, il suffit des fois de diluer l'anticorps primaire un peu plus.

J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres questions.

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Vielen Dank für Ihre Anfrage.

Frau Hoffmann ist im Moment im Urlaub und erst am Montag wieder verfügbar.

Leider bieten wir keinen Marker an, der höher als 225 kDa läuft. Allerdings habe ich einen Marker bis 300kDa bei Fermentas gefunden:


Da Sie ein sehr schweres Protein detektieren möchten, möchte ich Ihnen empfehlen, einen Ureapuffer zu verwenden. Ich habe Ihnen ein sehr interessantes Paper über die Löslichkeit von verschieden Proteinen hinsichtlich des Kompartiments und des Gewichtes an diese Email gehängt.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!

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