Product namePrestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa)
See all Prestained Protein Ladder reagents
DescriptionPrestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
Tested applicationsSuitable for: WB, SDS-PAGEmore details
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.5
Preservative: 0.01% Sodium azide
Constituents: 1.37% Tris phosphate, 0.03% EDTA, 2% SDS, 0.15% DTT, 33% Glycerol
Concentration information loading...
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Our Abpromise guarantee covers the use of ab234592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl
|SDS-PAGE||Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 ul per well for 15-well or 10-well mini-gel, respectively.
SDS-PAGE analysis with ab234592.
Gel 1: Tris-Glycine 4-20%
Gel 2: Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in diﬀerent electrophoresis conditions.
ab234592 has not yet been referenced specifically in any publications.