Overview

  • Product name

    Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa)
    See all Prestained Protein Ladder reagents
  • Tested applications

    Suitable for: WB, SDS-PAGEmore details
  • General notes

    Prestained Protein Ladder ab115832 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. This protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

    This product was previously called Prism Protein Ladder (10-175 kDa).

    Key product features:

    • Broad range: 10-175 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye.
    • Sharp bands

    Review other protein ladders in the unstained and prestained protein ladder guide.

     

     

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C short term (1-2 weeks). Store at -20°C.
  • Storage buffer

    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 0.01% Beta mercaptoethanol, 2% SDS, 15% Glycerol
  • Concentration information loading...
  • Research areas

  • Alternative names

    • Molecular weight marker
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder
    see all

Applications

Our Abpromise guarantee covers the use of ab115832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 - 5ul.
SDS-PAGE Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 ul per well for mini-gels and 10 ul for larger gels.

Images

  • SDS-PAGE with ab115832. Gel 1: Tris-Glycine 15%, Gel 2: Bis-Tris 4-12% MOPS buffer and Gel 3: Bis-Tris 4-12% MES Buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

References

ab115832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your patience. My colleague in the lab informed me that our optiblot range of protein molecular weight markers do contain His-tagged proteins. The markers ab115832 and ab48854 do not contain His-tagged proteins. I hope this helps, please let me know if you need any additional information or assistance.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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