Product namePrestained Protein Ladder - Mid-range molecular weight (10-175 kDa)
See all Prestained Protein Ladder reagents
Tested applicationsSuitable for: WB, SDS-PAGEmore details
Prestained Protein Ladder ab115832 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. This protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
This product was previously called Prism Protein Ladder (10-175 kDa).
Key product features:
- Broad range: 10-175 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye.
- Sharp bands
Review other protein ladders in the unstained and prestained protein ladder guide.
Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C.
Storage bufferConstituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 0.01% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 15% Glycerol
Concentration information loading...
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Our Abpromise guarantee covers the use of ab115832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 - 5ul.|
|SDS-PAGE||Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 ul per well for mini-gels and 10 ul for larger gels.|
SDS-PAGE with ab115832. Gel 1: Tris-Glycine 15%, Gel 2: Bis-Tris 4-12% MOPS buffer and Gel 3: Bis-Tris 4-12% MES Buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
ab115832 has not yet been referenced specifically in any publications.