Product namePrestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa)
See all Prestained Protein Ladder reagents
DescriptionPrestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
Tested applicationsSuitable for: WB, SDS-PAGEmore details
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 10-180 kDa
- Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.50
Constituents: 0.44% Tris phosphate, 2% SDS, 0.003% DTT, 21.6% Urea, 15% Glycerol
Concentration information loading...
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Our Abpromise guarantee covers the use of ab234617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 1.5-2.5 μl
|SDS-PAGE||Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 ul per well for 15-well or 10-well mini-gel, respectively.
ab234617 has not yet been referenced specifically in any publications.