Product namePrestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa)
See all Prestained Protein Ladder reagents
DescriptionPrestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
Tested applicationsSuitable for: WB, SDS-PAGEmore details
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 10-180 kDa
- Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.50
Constituents: 0.44% Tris phosphate, 2% Sodium lauryl sulfate, 0.003% DTT, 21.6% Urea, 15% Glycerol (glycerin, glycerine)
Concentration information loading...
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Our Abpromise guarantee covers the use of ab234617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 1.5-2.5 μl
|SDS-PAGE||Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 ul per well for 15-well or 10-well mini-gel, respectively.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234617 has not yet been referenced specifically in any publications.