Anti-PREX1 antibody (ab223698)
Key features and details
- Rabbit polyclonal to PREX1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PREX1 antibody
See all PREX1 primary antibodies -
Description
Rabbit polyclonal to PREX1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PREX1 aa 700-900.
Database link: Q8TCU6 -
Positive control
- IHC-P: Human hippopcampus, lymph node and small intestine tissues. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. -
Tissue specificity
Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues. -
Sequence similarities
Contains 1 DEP domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains. -
Cellular localization
Cytoplasm > cytosol. Cell membrane. Mainly cytosolic. Some amount is apparently associated to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 57580 Human
- Omim: 606905 Human
- SwissProt: Q8TCU6 Human
- Unigene: 721980 Human
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Alternative names
- 4 antibody
- 5)-dependent Rac exchanger 1 antibody
- KIAA1415 antibody
see all
Images
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Paraffin-embedded human hippocampus tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human lymph node tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human small intestine tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for PREX1 using ab223698 at 1/200 dilution in immunohistochemical analysis. Negative control - no staining.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PREX1 (green) using ab223698 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223698 has not yet been referenced specifically in any publications.