Anti-PREX2 antibody (ab169027)
Key features and details
- Mouse polyclonal to PREX2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PREX2 antibody
See all PREX2 primary antibodies -
Description
Mouse polyclonal to PREX2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein corresponding to amino acids 1-979 of Human PREX2 (AAI48796.1, Q70Z35-3).
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Positive control
- PREX2 transfected 293T cell line lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab169027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
Target
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Function
Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase. -
Tissue specificity
Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart. -
Sequence similarities
Contains 2 DEP domains.
Contains 1 DH (DBL-homology) domain.
Contains 2 PDZ (DHR) domains.
Contains 1 PH domain. -
Domain
PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.
DH domain alone was unable to confer substrate specificity and recognition. - Information by UniProt
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Database links
- Entrez Gene: 80243 Human
- Entrez Gene: 109294 Mouse
- Omim: 612139 Human
- SwissProt: Q70Z35 Human
- SwissProt: Q3LAC4 Mouse
- Unigene: 591867 Human
- Unigene: 123247 Mouse
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Alternative names
- 4 antibody
- 5)-dependent Rac exchanger 2 antibody
- 5-trisphosphate-dependent Rac exchanger 2 protein antibody
see all
Images
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Western blot - Anti-PREX2 antibody (ab169027)All lanes : Anti-PREX2 antibody (ab169027) at 1 µg/ml
Lane 1 : PREX2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 112 kDa
Datasheets and documents
References (5)
ab169027 has been referenced in 5 publications.
- Ai Y et al. Interference of P-REX2a may inhibit proliferation and reverse the resistance of SGC7901 cells to doxorubicin. Oncol Lett 15:3185-3191 (2018). PubMed: 29435055
- Horrigan SK et al. Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations. Elife 6:N/A (2017). PubMed: 28100394
- Yang J et al. PREX2 promotes the proliferation, invasion and migration of pancreatic cancer cells by modulating the PI3K signaling pathway. Oncol Lett 12:1139-1143 (2016). WB ; Human . PubMed: 27446408
- Dai Z et al. Knockdown of PREX2a inhibits the malignant phenotype of osteosarcoma cells. Mol Med Rep 13:1930-6 (2016). PubMed: 26718453
- Chroscinski D et al. Registered report: Melanoma genome sequencing reveals frequent PREX2 mutations. Elife 3:N/A (2014). PubMed: 25490935
