Anti-PREX2 antibody (ab169027)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-PREX2 antibody
See all PREX2 primary antibodies -
DescriptionMouse polyclonal to PREX2
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein corresponding to amino acids 1-979 of Human PREX2 (AAI48796.1, Q70Z35-3).
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Positive control
- PREX2 transfected 293T cell line lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab169027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
Target
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FunctionFunctions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
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Tissue specificityIsoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.
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Sequence similaritiesContains 2 DEP domains.
Contains 1 DH (DBL-homology) domain.
Contains 2 PDZ (DHR) domains.
Contains 1 PH domain. -
DomainPH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.
DH domain alone was unable to confer substrate specificity and recognition. - Information by UniProt
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Database links
- Entrez Gene: 80243 Human
- Entrez Gene: 109294 Mouse
- Omim: 612139 Human
- SwissProt: Q70Z35 Human
- SwissProt: Q3LAC4 Mouse
- Unigene: 591867 Human
- Unigene: 123247 Mouse
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Alternative names
- 4 antibody
- 5)-dependent Rac exchanger 2 antibody
- 5-trisphosphate-dependent Rac exchanger 2 protein antibody
see all
Images
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Western blot - Anti-PREX2 antibody (ab169027)All lanes : Anti-PREX2 antibody (ab169027) at 1 µg/ml
Lane 1 : PREX2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 112 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Ai Y et al. Interference of P-REX2a may inhibit proliferation and reverse the resistance of SGC7901 cells to doxorubicin. Oncol Lett 15:3185-3191 (2018). Read more (PubMed: 29435055) »
- Horrigan SK et al. Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations. Elife 6:N/A (2017). Read more (PubMed: 28100394) »
