Overview

  • Product name
    Anti-PREX2 antibody
    See all PREX2 primary antibodies
  • Description
    Mouse polyclonal to PREX2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein corresponding to amino acids 1-979 of Human PREX2 (AAI48796.1, Q70Z35-3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • PREX2 transfected 293T cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.

Target

  • Function
    Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
  • Tissue specificity
    Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.
  • Sequence similarities
    Contains 2 DEP domains.
    Contains 1 DH (DBL-homology) domain.
    Contains 2 PDZ (DHR) domains.
    Contains 1 PH domain.
  • Domain
    PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.
    DH domain alone was unable to confer substrate specificity and recognition.
  • Information by UniProt
  • Database links
    • Alternative names
      • 4 antibody
      • 5)-dependent Rac exchanger 2 antibody
      • 5-trisphosphate-dependent Rac exchanger 2 protein antibody
      • 6230420N16Rik antibody
      • DEP domain-containing protein 2 antibody
      • DEP.2 antibody
      • DEPDC2 antibody
      • FLJ12987 antibody
      • P-Rex2 antibody
      • Phosphatidylinositol 3 antibody
      • phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 antibody
      • phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein antibody
      • phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 antibody
      • PREX2 antibody
      • PREX2_HUMAN antibody
      • PtdIns(3 antibody
      • PtdIns(3,4,5)-dependent Rac exchanger 2 antibody
      see all

    Images

    • Western blot - Anti-PREX2 antibody (ab169027)
      Western blot - Anti-PREX2 antibody (ab169027)
      All lanes : Anti-PREX2 antibody (ab169027) at 1 µg/ml

      Lane 1 : PREX2 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Lysates/proteins at 15 µl per lane.

      Predicted band size: 112 kDa

    References

    This product has been referenced in:
    • Horrigan SK  et al. Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations. Elife 6:N/A (2017). Read more (PubMed: 28100394) »
    • Yang J  et al. PREX2 promotes the proliferation, invasion and migration of pancreatic cancer cells by modulating the PI3K signaling pathway. Oncol Lett 12:1139-1143 (2016). WB ; Human . Read more (PubMed: 27446408) »

    See all 2 Publications for this product

    Publishing research using ab169027? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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