Anti-PRG2 antibody [BMK13] (ab14462)
Key features and details
- Mouse monoclonal [BMK13] to PRG2
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-PRG2 antibody [BMK13]
See all PRG2 primary antibodies -
Description
Mouse monoclonal [BMK13] to PRG2 -
Host species
Mouse -
Specificity
Specific for eosinophil major basic protein (14kDa) irrespective of the stages of eosinophil activation and can be regarded as a “pan-eosinophil” marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kDa) and mature form found in the matrix of the eosinophil large specific granule (13.8kDa). The clone number has been updated from (1.B.787) to (BMK13) both clone numbers name the same antibody clone. -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Horse -
Immunogen
Full length protein (Human).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.065% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
BMK13 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (0.1% trypsin).
IHC-Fr: Use at an assay dependent dilution. Fix with methanol:acetone.
WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. -
Tissue specificity
High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Levels of the proform increase in serum and placenta during pregnancy. -
Post-translational
modificationsNitrated. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted. - Information by UniProt
-
Database links
- Entrez Gene: 5553 Human
- Omim: 605601 Human
- SwissProt: P13727 Human
- Unigene: 512633 Human
-
Alternative names
- BMPG antibody
- EMBP antibody
- Eosinophil granule major basic protein antibody
see all
Protocols
Datasheets and documents
References (0)
ab14462 has not yet been referenced specifically in any publications.