Anti-PRG2 antibody [BMK13] (ab14462)

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Overview

  • Product name
    Anti-PRG2 antibody [BMK13]
    See all PRG2 primary antibodies
  • Description
    Mouse monoclonal [BMK13] to PRG2
  • Host species
    Mouse
  • Specificity
    Specific for eosinophil major basic protein (14kDa) irrespective of the stages of eosinophil activation and can be regarded as a “pan-eosinophil” marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kDa) and mature form found in the matrix of the eosinophil large specific granule (13.8kDa). The clone number has been updated from (1.B.787) to (BMK13) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Horse
  • Immunogen

    Full length protein (Human).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.065% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    BMK13
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-Fr
IHC-P
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (0.1% trypsin).
    IHC-Fr: Use at an assay dependent dilution. Fix with methanol:acetone.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
    • Tissue specificity
      High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
    • Sequence similarities
      Contains 1 C-type lectin domain.
    • Developmental stage
      Levels of the proform increase in serum and placenta during pregnancy.
    • Post-translational
      modifications
      Nitrated.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted.
    • Information by UniProt
    • Database links
      • Alternative names
        • BMPG antibody
        • EMBP antibody
        • Eosinophil granule major basic protein antibody
        • Eosinophil major basic protein antibody
        • MBP antibody
        • MBP1 antibody
        • Natural killer cell activator antibody
        • Pregnancy-associated major basic protein antibody
        • PRG 2 antibody
        • PRG2 antibody
        • PRG2_HUMAN antibody
        • Proteoglycan 2 antibody
        • Proteoglycan 2 bone marrow antibody
        • Proteoglycan 2 preproprotein antibody
        see all

      References

      ab14462 has not yet been referenced specifically in any publications.

      Publishing research using ab14462? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      BATCH NUMBER 87396 ORDER NUMBER 1003198 DESCRIPTION OF THE PROBLEM No bands SAMPLE Human Endometrial Stromal Cells PRIMARY ANTIBODY Abcam - Mouse monoclonal [1.B.78] to PRG2 1 in 100 dilution Incubated overnight at 4 degrees Wash for 15 minutes DETECTION METHOD ECLPlus (Supersignal WestPico Chemiluminescent Substrate (Pierce) POSITIVE AND NEGATIVE CONTROLS USED None - can you recommend one? ANTIBODY STORAGE CONDITIONS 4 degrees SAMPLE PREPARATION RIPA lysis buffer (Upstate cell signalling solutions) Complete MINI Protease inhibitor (Roche diagnostics) AMOUNT OF PROTEIN LOADED 20 micrograms ELECTROPHORESIS/GEL CONDITIONS 4-15% gradient gel (BioRad ready gels 4-15% Tris-HCl) Reducing gel TRANSFER AND BLOCKING CONDITIONS Transfer for 30 mins at 15 volts. Buffer = Western Transfer Buffer (25mM Tris (3g/L), 0.2M glycine (15g/L), 20% methanol) Block in 5% milk solution for 30 minutes SECONDARY ANTIBODY Sheep Anti-Mouse IgG HRP conjugate [another supplier] 1 in 2500 Incubated for 1 hour at room temperature in 5% milk solution Wash for 40 minutes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Added more protein (originally added 10 micrograms)

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      Answer

      I have received confirmation from the laboratory that tested ab14462 that your protocol is very good and should work with ab14462. The laboratory shared also the following feedback which may interest you "Your customer's secondary antibody is a Sheep x mouse IgG HRP. Ab14462 is a mouse IgG1. I have had diverse experiences with this sort of distinction. I have had customers state that an anti-IgG will recognize all IgG's of the same species; and yet there have been times when I suggested matching the secondary precisely to the target isotype and suddenly the protocol was working." As the antibody did not work for you I would be happy to offer you a replacement vial or credit note/refund. Please let me know which you would prefer and I will arrange this, I look forward to hearing from you,

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      Question

      I have been using PRG2 antibody [1.B.787] in Western Blots, but have not had any success. Could you recommend a concentration of this antibody to use for direct ELISA?

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      Answer

      I have been informed by the source of ab14462 that it was not tested in house in ELISA and that unfortunately we therefore do not know the recommended dilution. I was recommended to start at a concentration of 2-5ug/ml (for Direct or Indirect ELISA), incubating overnight at 4C. I'm sorry I cannot provide further help. I will look into your complaint as soon as possible and appreciate your patience,

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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