Overview

  • Product name

    Anti-PRIC4/PRICKLE4 antibody
  • Description

    Rabbit polyclonal to PRIC4/PRICKLE4
  • Host species

    Rabbit
  • Specificity

    Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
  • Tested applications

    Suitable for: WB, ICC/IF, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PRIC4/PRICKLE4 (N terminal).
    Database link: NP_037529

  • Positive control

    • A549 cell lysate
  • General notes

     This product was previously labelled as PRIC4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 5 µg/ml.

Target

Images

  • Immunofluorescence of PRIC4/PRICKLE4 in A549 cells with ab119773 at 20 ug/mL.

  • All lanes : Anti-PRIC4/PRICKLE4 antibody (ab119773) at 0.25 µg/ml

    Lane 1 : A549 cell lysate without blocking peptide
    Lane 2 : A549 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 37 kDa

  • Immunocytochemical analysis of A549 cells, staining PRIC4/PRICKLE4 with ab119773 at 5 µg/ml.

References

This product has been referenced in:

  • MacNair L  et al. MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis. Brain 139:86-100 (2016). ICC/IF ; Mouse . Read more (PubMed: 26525917) »
See 1 Publication for this product

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